摘要
目的 探讨从牛舌黏膜上皮中提取寻常型天疱疮抗原的可行性及其在天疱疮研究中的意义。方法 应用8mol/L尿素浸泡法制备牛舌黏膜上皮提取物，免疫印迹分析寻常型天疱疮患者血清中天疱疮抗体结合的靶抗原。 结果 13例寻常型天疱疮全部检出IgG型和IgA型天疱疮抗体,它们分别识别牛舌黏膜上皮提取物中260 ku、230 ku、210 ku、190 ku、165 ku、160 ku、135 ku、115 ku、97 ku、85 ku、65 ku和62 ku多肽中一种或几种抗原。 结论 从牛舌中提取寻常型天疱疮抗原的方法可行，适合于天疱疮抗体的免疫印迹检测。
Objective To explore the feasibility of the methods e xtracting pemphigus vulgaris antigens from bovine tongue and its value in the study on pemphigus. Methods The extracts were prepared by 1 mol/L sodium chloride-separated mucosa of bovine tongue and soaking in 8 mol/L urea, and reacted with sera from patients with pemphigus vulgaris(PV) by immunoblot. Results One or more moleculars of 260 ku, 230 ku, 210 ku, 190 ku, 165 ku, 160 ku, 135 ku, 115 ku, 97 ku, 85 ku, 65 ku and 62 ku polypeptides in the extracts were recognized by 13 PV sera. Conclusion The methods extracting PV antigens are practical, and suitable for the detection of pemphigus antibodies.
出处
《安徽医科大学学报》
CAS
2001年第5期348-350,共3页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金资助课题(编号:97424005)
关键词
天疱疮
免疫学
抗原
免疫印迹法
牛舌粘膜
pemphigus/immunology
antigens
immunoblotting Freewords: bovine tongue
