摘要
目的 探讨高压氧（ｈｙｐｅｒｂａｒｉｃ ｏｘｙｇｅｎａｔｉｏｎ，ＨＢＯ）对移植肾慢性排斥反应的防治作用及其机制。方法 以２２只雌性Ｆ３４４大鼠为供体，２２只雌性Ｌｅｗｉｓ大鼠为受体，应用显微外科技术制作移植肾慢性排斥反应大鼠模型，将受体大鼠随机分为治疗和对照两组。治疗组 １１只接受 ＨＢＯ治疗，治疗压力为 ０．２ ＭＰａ，每天 ４０ ｍｉｎ；对照组 １１只不接受 ＨＢＯ治疗。移值后 ２４周处死动物，对移植肾组织行肾功能、组织学、免疫组化和反转录聚合酶链反应（ＲＴ－ＰＣＲ）检测。结果 对照组蛋白尿明显多于治疗组，肌酥清除率明显低于治疗组，肾间质纤维化、肾小球硬化、动脉血管内膜增厚较治疗组严重。治疗组移植肾Ｔ细胞、单核／巨噬细胞浸润较对照组轻，但无统计学意义；而ＰＤＧＦ－ＡＡ表达水平较对照组明显低，其差异有统计学意义。结论ＨＢＯ在预防或减轻同种移植肾慢性排斥反应中能起重要的作用。
Objective To explore the efficacy of hyperbaric oxygenation (HBO) on preventing renal allograft chronic rejection. Methods 22 female Lewis rats received kidney transplantation from fe-male F344 donors. Micro-surgery technique was used in transplant operation. All of the recipients were divided into two groups. 11 recipients were treated with HBOCO. 2 MPa 40 min each day) as treatment group. Other 11 recipients with no treatment as control group. 24 weeks after transplantation, all re-cipients were killed and renal functions were evaluated. Histologic, immunohistological and RT-PCR analysis were performed in kidney allografts. Results Compared to treatment group, the controls had significantly worse proteinuria; lower creatinine clearance rate; more severe tubulo-interstitial fibrosis and infiltration, glomerulosclerosis and arterial intima thickening. The infiltration of T-cells and mono-cytes/macrophoges in the allograft kidney of treatment group was less than that in the controls but without stat istical significance. More expression of PDGF-AA mRNA existed in the controls and was statistically significant as compared to treatment group. Conclusions HBO could play important role in preventing or reducing chronic allograft rejection.
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
2001年第3期164-166,共3页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
关键词
高压氧
移植肾
慢性排斥反应
治疗
Hyperbaric oxygenation Kidney allograft Chronic rejection
