摘要
目的 :研究乙型肝炎病毒 (HBV)的子宫内传播率与受染子代的 HBV的肝外定位。方法 :选择 2 5名女性 HBV携带者与其终止妊娠的胎儿为研究对象 ,应用 HBV S基因与 C基因引物 ,以巢式 PCR检测 HBV DNA。结果 :2 5名女性携带者的胎儿中 10例发生 HBV的子宫内传播 ,传播率为 40 .0 % ,受染子代的肝外 HBV DNA检出率以白细胞为最高 ,其次脾脏、肺脏、心脏 ,此外从肾脏、脐带、皮肤、肌肉等多种组织、器官也可检出。结论 :HBV可在肝外多种组织、器官定位 。
Objective:The study was carried out,to study transmission of HBV in womb and HBV in tissues out of liver.Methods:The study contained 25 HBV women carriers and their foetus.HBV DNA was detected by nest PCR and the primers were from HBV S gene,C gene.Results:10 of 25 foetus were infected,the rate of HBV transmission in womb was 40 0%.HBV DNA was detected in peripheral blood leukocyte,spleen,lung,heart,kidney,umbilical cord,skin,muscular,etc.The rate of HBV detected in peripheral blood leukocyte was more high than other tissues.Conclusion:HBV may be detected in varied tissues or organs.HBV in tissues out of liver may be helpful to preserve virus.
出处
《现代预防医学》
CAS
2001年第3期267-268,共2页
Modern Preventive Medicine
