摘要
目的 :研究子宫内感染的 HBV基因前 C/ C区变异株。方法 :以 10名女性 HBV携带者及其中终止妊娠的 10例子宫内感染的胎儿为研究对象。以 PCR及错配 PCR扩增 HBV DNA,限制片段长度多态性分析 (RFL P)检测前 C区终2 8变异和 C区 L 97变异。阳性者再以双脱氧链末端终止法证实。结果 :10例胎儿及母亲中 ,1例胎儿与母亲检出 L 97变异株与原型株的混合感染。其他 9例胎儿及母亲均为原型株。终 2 8变异株均未检出。结论 :HBV子宫内传播以母亲携带的优势株为主。 RFL P是检测 L 97、终 2 8两个变异热点的简便方法。
Objective:The study on HBV precore C/C gene variations of infection in the womb.Methods:This paper was consisted of 10 female HBV carriers and 10 fetus who were infected HBV in the womb.PCR and mispairing PCR produced DNA fragments.The variations of stop 28 at Pre C gene and L97 at C gene were detected by restriction fragment length polymorphism analysis(RFLP).The PCR products which were positive by RFLP were directly sequenced.Rusults:1 pair mother and fetus were infected with L97 variation strain and wild strain.Other 9 pairs were infected with wild strain.The stop 28 variation was negative in 10 pairs.Conclusion:HBV infection in the womb was proponderant virus strain of mother.RFLP was a simple method which detected stop 28 and L97 variation.
出处
《现代预防医学》
CAS
2001年第1期4-6,共3页
Modern Preventive Medicine
