摘要
我国浙江、江苏、四川等省发生的小麦土传病毒病,由禾谷多粘菌(Polymyxa graminis)传播,只感染小麦,感病植株幼叶表现为退绿到黄化的条斑,老叶表现为花叶和坏死。我们提纯各地分离物研究表明,病毒粒子呈线状,直径13～14nm,长度为200～1800nm,其中350～850nm的比例较高。病毒外壳由二种分子量分别约为30kd和27kd的结构蛋白组成。病毒粒子周围能均匀地“修饰”小麦梭条斑花叶病毒(WSSMV)抗血清和小麦黄花叶病毒(WYMV)抗血清,反应均很强烈。鉴于上述特性,认为本病害是由小麦棱条斑花叶病毒(WSSMV)引起的。
A soil-borne virus disease on wheat occured in Zhejiang, Jiangsu and Sichuan provinces in China was transmitted by Polymyxa graminis, and its only known host is wheat (Triticum aestivum), most wheat cultivars can be infected by growing in infective soil with chlorotic to necrosis spindle shaped streak in the leaves. Studies on virus isolates from different disesed areas showed that virus particles is filamentous, with measurements ranging from 200—1800×13—14 nm, mostly 350—850×13—14 nm, in leaf-dip or purified preparations. Coat proteins of all virus isolates electrophoresised in SDS-polyacry-lamide gel migrated as two bands with moleclar weight of 30 and 27 KD respectively. The virions decorated strongly with antiserum against to WSSMV isolate and to Japanese WYMV isolate were observed on screen by Immunosorbent Electron Microscopy(ISEM). On the basis of these properties, we consider that the pathogen of the disease is wheat spindle streak mosaic virus(WSSMV).
出处
《病毒学杂志》
CSCD
1989年第2期176-181,共6页
基金
浙江省自然科学基金
关键词
小麦
土传病毒病
病原
Wheat soil-borne virus disease,Pathogen, Particle length,Coat protein,Immunosorbent electron microscopy(ISEM), Wheat spindle streak mosaic virus(WSSMV)
