摘要
根据已发表的日本小麦黄花叶病毒（ｗｈｅａｔｙｅｌｏｗｍｏｓａｉｃｂｙｍｏｖｉｒｕｓ，ＷＹＭＶ）ＲＮＡ１核苷酸序列，设计了一对引物ＮＩｂ０１／ＮＩｂ０２，用来扩增浙江浦江土传线状小麦黄花叶病毒分离物ＮＩｂ基因的全长序列。以表现典型病症的小麦植株病叶总ＲＮＡ为模板，Ｏｌｉｇｏｄ（Ｔ）１８为引物合成第一链ｃＤＮＡ，用ＲＴＰＣＲ法扩增浦江分离物ＮＩｂ全长基因，并将此基因直接克隆到ｐＧＥＭＴ质粒中，进行全序列测定。结果表明该分离物与日本ＷＹＭＶ相应序列具有很高的同源性，而与小麦梭条斑花叶病毒（ＷＳＳＭＶ）存在很大的差异，因此认为浦江病毒分离物为ＷＹＭＶ。
A 1.6 kb fragment,which contains complete sequence of NIb gene of a soilborne wheat bymovirus,collected from infected field in Pujiang,Zhejiang Province,was amplified by RTPCR with a pair of primers (NIb01 and NIb02),which were designed according to the published sequences of Japanese wheat yellow mosaic bymovirus(WYMV),cloned and sequenced.The sequence comparison indicated that the homology of this NIb gene to Japanese WYMV isolates was very high (99.1% identity in nucleotides),but the homology with other bymoviruses was 73.1%-74.4% (nucleotides).The results confirmed that the virus in Pujiang was indeed wheat yellow mosaic virus (WYMV),rather than WSSMV.
出处
《浙江农业学报》
CSCD
1999年第3期109-113,共5页
Acta Agriculturae Zhejiangensis
基金
欧共体
863项目资助
