摘要
目的对TTV阳性扩增产物进行克隆及测序,以了解西安地区TTV基因型及基因结构的特点。方法采用巢式PCR从转氨酶活性异常增高的患者血清中,得到TTV阳性扩增产物,将其插入pGEM-Teasy载体,进行克隆及序列分析。结果获得1个pGEM-TTV重组载体。序列分析表明,插入片段为196bp。该序列与AF065400株等对应位置的核苷酸同源性分别为:AF065400中国株94.9%、AF351132中国株98.0%和NC-002076日本株99.0%。结论西安地区TTV阳性扩增序列与报道的AF065400株、AF351132株和NC-002076株的序列具有高度的同源性,属同个基因型别。
Aim To clone and sequence specificaeey a mplified products of TT Virus,so as to umderstand the genotype and gene structure novel virus from Xi ' an area.Methods By nested -PCR,the presence of TTV DNA in serum samples from patient s with elevated transaminase levels were examined by nested -PCR t he presence of TTV DNA.The PCR product was cloned into Easy Vect or pGEM -T and then sequence analysis was performed.Results DNA sequence analysis showed that th e length of insert seqment was 1966.The sequence hak a striking ho-mology to reported three sequences,AF065400(from China),AF351132(from China)and NC -002076(from Japan)nucleotide homology on the corresponding sites to above 3sequences were 94.9%,98.0%and99.0%,respectively.Conclusion Amplified sequence of TTV from xi' an area had striking sequence homologies to reported three strains(i.e.,AF065400,AF351132and NC -002076)belong to the same genotype.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2001年第5期441-442,共2页
Chinese Journal of Cellular and Molecular Immunology
