摘要
目的：了解接种患者血清的恒河猴能否复制 Ｔ Ｔ 病毒（ Ｔ Ｔ ｖｉｒｕｓ， Ｔ Ｔ Ｖ），正常恒河猴中是否有 Ｔ Ｔ Ｖ 感染，分析猴中 Ｔ Ｔ Ｖ 基因结构特点。方法：用 Ｐ Ｃ Ｒ 方法检测血清标本中的 Ｔ Ｔ Ｖ Ｄ Ｎ Ａ，对 Ｔ Ｔ Ｖ Ｄ Ｎ Ａ 阳性的标本进行序列测定。结果：健康猴中无 Ｔ Ｔ Ｖ 感染；１０ 只接种患者血清的恒河猴中，经检测有 ４ 只为 Ｔ Ｔ Ｖ 阳性。将其中一株 Ｔ Ｔ Ｖ 进行序列测定，该序列与日本 Ｔ Ｔ Ｖ 部分基因相对应位置的核苷酸同源性为 ９６％ ，与中国株 Ｔ Ｔ Ｖ 全基因相对应位置的核苷酸同源性为 ９８％ 。结论：健康猴群中无 Ｔ Ｔ Ｖ 自然感染。恒河猴可感染 Ｔ Ｔ Ｖ，可作为研究 Ｔ Ｔ Ｖ 的动物模型。
Objective: To learn whether natural infection of TTV exists in Macaca mulatta or not, and to investigate the replication of TTV in Macaca mulatta inoculated with serum of patients with non A E hepatitis. Methods: Serum samples collected from inoculated and normal monkeys were examined for TTV DNA using PCR method and the PCR products were sequenced. Results and Conclusion: TTV DNA was positive in 4 of 10 inoculated monkeys and none in normal monkeys. The partial gene of TTV was cloned and sequenced. The partial gene of TTV isolated from monkey showed 96% nucleotide identity with the corresponding one of TTV isolated in Japan, and 98% identity with TTVCH1(isolated in China).
出处
《军事医学科学院院刊》
CSCD
北大核心
1999年第3期187-189,共3页
Bulletin of the Academy of Military Medical Sciences
基金
军事医学科学院 1997 年度创新基金
关键词
恒河猴
TT病毒
聚合酶链反应
实验研究
Macaca mulatta
TT virus
polymerase chain reaction
DNA sequencing
