摘要
分析和讨论了中国人庚型肝炎病毒（HGV）的基因结构特点及我国HGV感染的地理分布状况。中国庚型肝炎病毒株（HGVC964）基因全长9128个核苷酸，编码一个长度为2870个氨基酸残基的多聚前体蛋白。5’端有一个367bp的非翻译区，3’端非编码区为147个核苷酸。基因组中G＋C占59．9％。该株病毒与国外报道的三株HGV序列比较，核苷酸同源性为82．0％～86．2％，氨基酸同源性均大于90％。对我国部分地区的某些人群进行的分子流行病学研究表明，在我国北方、南方及广西少数民族地区也存在HGV感染。HCV自然感染者中，HGVRNA阳性率为6．7％；HC病人中，HGVRNA阳性率为9．6％；非甲一非戊型肝炎病人中，HGVRNA阳性率为5．0％。这表明，在我国HGV感染分布广泛，不同地理区带均有可能存在该型病毒感染。
The full HGV genome from Chinese was cloned and sequenced from the serum of ablood donor in Hebei province of China. The sequence of 9128bp indicatal that it contains a continuous open reading frame (ORF) which could encode a viral polyprotein of 2870 amino acids.The 5' and 3' untranslated regions are 367bp and 147bp, respectively Compared with publishedHGV sequences , Chinese HGV shows 84% ～ 89% identity at the nucleotide, and 91%～ 94% atthe amino .acid, levels.In order to understand the distribution of HGV infections and molecular characteristics ofHGV isolates from China, serum samples were detected by RT- PCR for HGV RNA and.the PCR products,were sequenced. The serum samples were collected from Hebei province, Nanjing, Guangxi andShanghai city, respectively, The results showed that HGV infection existed both in northern andsouthern China. The positive rate of HGV RNA in population with HCV infection was about6.7%, the positive rate. in patients with hepatitis C was 9.6% and the positive rate in blood donorswas 2.2%. The nucleotide identity between the two isolates (Nanjing and Hebei isolate) is 93.7%,while the two isolated showed 86.06～ 92.12% nucleotide identity over the corresponding region ofisolates in America.
出处
《微生物学通报》
CAS
CSCD
北大核心
1997年第1期27-31,共5页
Microbiology China
基金
"九五"全军医药卫生科学研究基金
关键词
庚型肝炎病毒
基因结构
感染
地理分布
Hepatitis G virus, cDNA sequencing, RT-PCR, Geographic distribution
