期刊文献+

结球白菜组培再生苗玻璃化问题初步研究 被引量:10

Vitrification of Regeneration Shoots of Chinese Cabbage
在线阅读 下载PDF
导出
摘要 从培养基组份和培养条件两方面对结球白菜玻璃化的成因进行了研究 .初步认为激素浓度和琼脂浓度是影响结球白菜玻璃化的主要原因 .在一定范围内 ,降低BA、NAA浓度、提高琼脂浓度 ,不会影响芽的分化率 ,却有利于正常芽的分化形成 ;而改用通气性好的三角瓶或降低AgNO3 浓度 。 Medium gradient and culture conditions in plant tissue culture of Chinese cabbage were studied.The vitrification of regeneration shoots of Chinese cabbage in vitro was mostly affected by hormones concentration and agar concent.To some certain,the normal shoot regeneration was facilitated by decreasing hormone concentration of increasing agar concentration.But there is no evident effect for inhibiting the occurrence of vitreous shoot by decreasing AgNO\-3 concentration or changing culture dishes into culture flasks.
出处 《首都师范大学学报(自然科学版)》 2001年第3期54-57,64,共5页 Journal of Capital Normal University:Natural Science Edition
基金 北京高技术实验室科研项目资助课题
关键词 结球白菜 玻璃化 组织培养 再生苗 激素浓度 琼脂浓度 分化率 Chinese cabbage, vitrification, thssue culture
  • 相关文献

参考文献14

  • 1卜学贤 陈维伦.试管植物的玻璃化现象[J].植物生理学通讯,1987,(5):13-18.
  • 2程振东,卫志明,许智宏.根癌农杆菌对甘蓝型油菜的转化及转基因植株的再生[J].Acta Botanica Sinica,1994,36(9):657-663. 被引量:94
  • 3刘非燕,郭达初.重瓣丝石竹试管苗玻璃化发生原因初探[J].杭州大学学报(自然科学版),1996,23(4):382-387. 被引量:21
  • 4张洪胜 牟云官 辛培刚.苹果离体培养中试管苗玻璃化现象发生机理探讨[J].林业科学,1992,17(3):10-12.
  • 5Bottcher C H, Vogelmann T C. Effect of righdity of gel medium on benzyladenine-induced adventitious bud formation and vitrification in Picea abics. Physiology Plant, 1984,61:505~512
  • 6Zim M, Meir G, Harlevy A. Factors influencing the production of harded glaucous Carnation plantlets in vitro. Plant Cell,Tissue and Organ culture, 1982,2(1):55~65
  • 7Leshem B, Shaley D P, Izhar S. The effect of cytokinins on vitrification in melon and carnation. Annals of Botany,1988,62(2):272~276
  • 8朱青松,梅康凤,王沙生.外源生长素对烟草髓愈伤组织分化和内源IAA含量的影响[J].北京林业大学学报,1999,21(1):22-25. 被引量:28
  • 9Chi G L, Pua E C, Goh C J, et al. Role of ethylene on de novo shoot regeneration from cotyledon explants of BassiBca campestris ssp. pekinensis(Lour) Olsson in vitro. Plant Physiol, 1991,96:170
  • 10F L Zhang, Y Takahata, J B Xu. Medium and genotype factors influencing shoot regeneration from cotyledonary explants of Chinese cabbage(Brassica campesstris L. ssp. pekinensis). Plant Cell Reports, 1998,17:780~786

二级参考文献32

共引文献439

同被引文献139

引证文献10

二级引证文献98

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部