摘要
研究了氮源对隐甲藻( Crypthecodinium cohnii )生长及产量的影响。结果表 ATCC30556 DHA 明:采用单一氮源,胰蛋白胨和蛋白胨有利于隐甲藻生长,在总氮量浓度下,隐甲藻生物量分别 0.34 g/L 达到 2.78 g DW和/L 2.70 g DW。蛋白胨培养时产量最高,为。保持总氮量不变,采用蛋/L DHA 338.56 mg/L白胨和硝酸钾为复合氮源,当混合氮源中蛋白胨:硝酸根为:时,隐甲藻有着最大的生物量和-N-N 12 产量,分别为DHA 2.98 gDW和。/L 527.97
The effect of various nitrogen sources on the growth and DHA yield is determined in Crypthecodinium cohnii ATCC30556. Single nitrogen tryptone and peptone are suitable to the growth, the biomass up to 2.78 g DW/L and 2.70 gDW/L respectively on the medium containing 0.34 g/L nitrogen. Peptone is a favorable nitrogen source for DHA accumulation, and DHA yield increases up to 338.56 mg/L. Using peptone and KNO3 as multiple nitrogen source, the maximum biomass and DHA concentrations are obtained from media containing 0.34 g/L nitrogen when the ratio of :peptone-NNO3:-N is 12, and the values are 2.98 gDW/L and 527.97 mg/L respectively.
出处
《海洋通报》
CAS
CSCD
北大核心
2001年第5期18-23,共6页
Marine Science Bulletin
基金
广东省自然科学基金(970486 )资助项目
