摘要
目的 研究苦参碱对人白血病细胞K562增殖、凋亡的影响及其机制。方法 苦参碱（0．2 mg/ml）作用于K562细胞，每日计数白细胞、计算抑制率；流式细胞分析仪检测苦参碱对K562细胞周期的改变及凋亡的影响；电子显微镜观察凋亡细胞形态；Western blot检测BCL-6及增殖细胞核抗原（PCNA）表达情况。结果 苦参碱对K562细胞有明显的抑制作用，用药72 h的细胞增殖抑制率达69%；用药5 d后，G1期细胞明显增多，高达58．5%，68%的细胞发生凋亡。电子显微镜可观察到苦参碱诱导的早、中、晚期K562凋亡细胞；BCL-6表达增强，PCNA表达下降。结论 苦参碱能显著抑制K562细胞增殖、促进其凋亡，其机制可能与细胞增殖周期受阻及PCNA表达下降、BCL-6表达增加有关。
Objective To study the effects of matrine on proliferation and apoptosis of K562 cells． Methods Cell culture and cell count, cell cycle phases and number of apoptotic cells were analyzed by flow cytometry． Morphology of apoptotic cells was studied by electron microscopy, BCL-6 and proliferating Cell nuclear antigen(PCNA)were checked by Western blot． Results Matrine could inhibit the proliferation of K562 cells with inhibition rate of 69% after 72 hours matrine induction； number of K562 cells increased significantly in G1 phase, and number of apoptotic cell was 68% at 5th day of matrine treated cells； the typical morphology of apoptotic cells of K562 cells was observed by electron microscopy； BCL-6 was increased and PCNA was decreased in K562 cells induced by matrine． Conclusion Matrine inhibited the proliferation and induced the apoptosis of K562 cells significantly, the mechanism of which might be related to cell cycle arrest and the change of PCNA and BCL-6．
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2001年第4期333-336,共4页
Acta Academiae Medicinae Sinicae
基金
第四军医大学创新工程( CX01A004)资助
关键词
苦参碱
K562细胞
凋亡
增殖
matrine； K562 cells； apoptosis； proliferation
