摘要
目的:研究苦参碱对体外培养人肺癌A549细胞的抑制作用及其机制。方法:用不同浓度的苦参碱作用A549细胞24、48、72h,以MTT法检测苦参碱对A549细胞的抑制作用,流式细胞仪检测凋亡率和细胞周期分布时相,TRAP-ELISA法检测端粒酶的活性。结果:随着时间和浓度的增加,苦参碱对A549细胞的抑制作用逐渐增强(P<0.01);不同浓度苦参碱(0.5、1.0、1.5、2.0mg/mL)诱导A549细胞的凋亡率分别为3.2%、9.5%、13.4%、17.6%;G0/G1期细胞比例增加,G2/S期细胞比例下降;端粒酶的活性呈浓度和时间依赖性降低。结论:苦参碱可通过抑制端粒酶活性、诱导细胞凋亡抑制体外培养的A549细胞的增殖。
Objective To explore the inhibitory effect of matrine on human lung carcinoma A549 cells in vitro and its mechanism. Methods A549 cells were treated with different concentrations of matrine solution for 24, 48 and 72 h, respectively. MTr assay was used to evaluate the cytotoxic effect of martrine on A549 cells. The apoptosis and cell cycle distribution of A549 cells were detected by flow cytometry and telomerase activity was determined by TRAP-ELISA methods. Results With the increase of the concentration of matrine solution and the duration of administration, the cytotoxic effect of matrine was elevated （ P 〈 0.01 ） . The apoptosis rate was 3.2%, 9.5%, 13.4%, and 17. 6% when A549 cells were treated with matrlne solution at 0. 5, 1.0, 1.5, and 2. 0 mg/mL, respectively. The proportion of cells in G0/Gi phase was obviously increased whereas that in C2/S phase was decreased. Telomerase activity was reduced in a time- and dose-dependent manner. Conclusion Martrine can inhibit the proliferation of A549 cells in vitro by inhibiting telomerase activity and inducing apoptosis.
出处
《实用医学杂志》
CAS
2006年第15期1717-1719,共3页
The Journal of Practical Medicine
基金
十堰市科技攻关项目(编号:2005ZD017)
关键词
肺肿瘤
苦参碱
细胞凋亡
端粒酶
Lung neoplasms Martrine Apoptosis Telomerase
