摘要
目的 探讨严重烧伤早期心肌线粒体F0 F1 ATPase活性变化及其对能量代谢的影响。方法 复制 3 0 %TBSAⅢ度烧伤大鼠模型 ,取正常及伤后 1、3、6、12、2 4h大鼠心肌分离线粒体 ,测定F0 F1 ATPase活性、线粒体膜电位及ATP、ADP、AMP含量。结果①烧伤后 1h线粒体F0 F1 ATPase合成活性明显升高 ,但 3、6、12、2 4h显著降低 ,分别为对照组的 5 1.4%、44.9%、77.6%和 87.4%。F0 F1 ATPase水解活性于伤后 1h明显降低 ,但 3h显著高于对照组 ,6、12、2 4h下降 ,明显低于正常对照组 ;②大鼠烧伤后 1h心肌线粒体ATP含量及膜电位均无明显变化 ,但 3、6、12、2 4h线粒体ATP含量及膜电位均显著降低 ,同时ADP含量升高。结论 烧伤后1h线粒体F0 F1 ATPase合成活性明显升高 ,加速ATP合成以满足细胞对能量的需求 ;伤后 3hF0 F1 ATPase水解活性明显升高 ,水解ATP以维持线粒体膜电位 ;6hF0 F1 ATPase功能紊乱 ,合成与水解活性均显著降低 ,失去其对能量代谢的调节作用。 12、2 4hF0 F1 AT Pase合成与水解活性均有所恢复 ,线粒体ATP含量及膜电位也呈恢复趋势。
Objective To explore the changes of myocardial mitochondrial F 0F 1 ATPase activity and its effects on energy metabolism in early stage of severe burns. Methods Myocardial mitochondria were isolated from normal control and burned rats(30%TBSA Ⅲ) at the 1st, 3rd, 6th, 12th and 24th hour postburn. The mitochondrial F 0F 1 ATPase activity, transmembrane potential and content of ATP, ADP and AMP were determined. Results ①The F 0F 1 ATPase synthetic activity increased at the 1st hour postburn, and decreased to 51.4%, 44.9%, 77.6% and 87.4% of that of the control at the 3rd, 6th, 12th and 24th hour postburn respectivly. The F 0F 1 ATPase hydrolytic activity decreased at the 1st hour postburn and was higher at the 3rd when compare with the normal control, it decreased again at the 6th, 12th and 24th hour postburn. ②No significant changes were found in the mitochondrial ATP content and transmembrane potential at the 1st hour postburn, however both of them decreased at the 3rd, 6th, 12th and 24th hour postburn, only the ADP content increasedo. Conclusions At the 1st hour postburn, myocardial mitochondrial F 0F 1 ATPase reinforces ATP production with higher synthetic activity, at the 3rd hour, it reinforces ATP hydrolysis with higher hydrolytic activity to maintain the mitochondrial transmembrane potential, at the 6th hour, F 0F 1 ATPase loses its ability in regulation of energy metabolism with lower synthetic and hydrolytic activity, and at 12th, 24th hour postburn, its synthetic and hydrolytic activity, mitochondrial ATP content and transmembrane potential all show a tendency to recover.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第7期780-782,共3页
Journal of Third Military Medical University
基金
国家重点基础研究发展规划项目基金资助 ("973"项目 )(G19990 54 2 0 2 )
