摘要
目的 探讨严重烧伤早期心肌线粒体Ca2 +转运变化及其发生机制。方法 复制 30 %Ⅲ度烫伤大鼠模型 ,测定伤后1、3、6、1 2、2 4h大鼠心肌线粒体Ca2 +转运速率 ,同时检测影响Ca2 +转运的相关指标———心肌细胞胞浆Ca2 +浓度 ( [Ca2 +]c)、线粒体膜电位及ATP含量。结果 伤后 1h心肌线粒体Ca2 +摄取速率明显升高 ,而Ca2 +释放速率无明显改变 ,但 3、6、1 2、2 4h心肌线粒体Ca2 +摄取与释放速率、线粒体膜电位、ATP含量均显著降低 ,且烧伤后线粒体Ca2 +摄取速率与膜电位呈明显正相关 ,Ca2 +释放速率与线粒体ATP含量呈显著正相关。而伤后 3、6、1 2、2 4h心肌 [Ca2 +]c均明显高于正常对照组。结论 ①烧伤初始心肌线粒体Ca2 +摄取加强 ,伤后续阶段线粒体Ca2 +转运紊乱 ;②线粒体膜电位下降、ATP含量降低是烧伤后续阶段心肌线粒体Ca2 +转运紊乱的主要原因 ,伤后 [Ca2 +]c升高或有升高趋势也参与严重烧伤早期心肌线粒体Ca2 +转运变化的发生。
Objctive To investigate the changes of myocardial mitochondrial Ca 2+ transport and its mechanism in the early stage after severe burns. Methods An experimental model of 30%TBSA full thickness skin scalding was prepared in rats. Mitochondrial Ca 2+ transport velocity were measured 1, 3, 6, 12 and 24 h after burns. The other 3 parameters which affecting the velocity were determined, i.e. membrane potential, ATP content and cytosolic Ca 2+ concentration ([Ca 2+ ]c). Results ①Mitochondrial Ca 2+ uptake velocity of the 1st hour postburn was higher than that of the control, and Ca 2+ release velocity didn't change obviously, but both of them were decreased at the 3rd, 6th, 12th, 24th hour postburn. ②Mitochondial membrane potential and ATP content descened at the 3rd, 6th, 12th, 24th hour postburn. ③Mitochondrial Ca 2+ uptake velocity was positive related with membrane potential after burns, and Ca 2+ release velocity was positive related with mitochondrial ATP content too. ④[Ca 2+ ]c was increased at the 3rd, 6th, 12th, 24th hour postburn. Conclusion ①There was an increase of mitochondrial Ca 2+ uptake at the beginning of burns, and obvious Ca 2+ transport disorder in myocardial mitochondria in the following stages. ②decline of ATP and membrane potential cause the disorder of myocardial mitochondrial Ca 2+ transport in the following stage after burns. The increase of [Ca 2+ ]c or the tendency is involved in the changes of myocardial mitochondrial Ca 2+ transport postburn.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第1期45-47,共3页
Journal of Third Military Medical University
基金
国家"973"重点基础研究发展规划项目基金资助 !(G1 9990 542 0 2 )
关键词
烧伤
膜电位
心肌线粒体
钙离子
钙转运
burn
mitochondria
calcium
myocardium
membrane potential
