摘要
目的 :探讨喉鳞状细胞癌端粒酶活性表达及其与颈淋巴结转移的相关性。方法 :采用多聚酶链反应 -酶联免疫吸附法 (PCR- EL ISA) ,对 47例喉鳞状细胞癌组织及 10例声带炎性息肉组织的端粒酶活性进行定量检测。结果 :47例喉鳞癌组织中 39例端粒酶呈阳性 (83% ) ,而 10例声带炎性息肉组织均为阴性 ,两组差异有极显著性意义 (P <0 .0 1) ;端粒酶活性在伴有颈淋巴结转移的喉癌中显著高于无淋巴结转移者 (P <0 .0 5 )。结论 :端粒酶在喉癌组织中有较高的表达 ,可作为喉癌的肿瘤标志物。喉癌组织端粒酶活性有可能作为预测喉癌转移和预后的指标 ,并可望指导临床有针对性地应用选择性颈廓清术。
Objective:To investigate the telomerase activity in human laryngeal squamous cell carcinomas (LSCC) and the association between telomerase activity and lymphatic metastasis.Method:Telomerase activity was determined in 47 specimens of LSCC and 10 specimens of laryngeal inflammatory polyps using PCR ELISA.Result:Telomerase activity was detected in 39 of 47 (83%) LSCC,but was not detected in all 10 laryngeal inflammatory polyps.There were significant differences between them (P< 0.01 ).Telomerase activation in LSCC with positive cervical lymph node involvement appeared to be higher (13 out of 13 cases,100%) than in LSCC with negative cervical lymph node involvement (26 out of 34, 76.47 %),(P< 0.05 ).Conclusion:Telomerase is activated frequently in LSCC and may become a biomarker of LSCC.The detection of telomerase activity in LSCC may have some value for predicting metastasis potential and prognosis and can guide the clinical practices applying selective neck dissection.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2001年第8期360-362,共3页
Journal of Clinical Otorhinolaryngology
基金
安徽省卫生厅资助项目 (No.A95 - 1998- 2 5 )
关键词
喉肿瘤
端粒酶
淋巴转移
聚合酶链反应
酶联免疫吸附测定
Laryngeal neoplasms
Telomerase
Lymphatic metastasis
Polymerase chain reaction
Enzyme linked immunosorbent assay
