摘要
目的:定量检测细胞周期素E(Cyclin E)在喉鳞状细胞癌及颈部淋巴组织中的表达,探讨其在喉癌转移中的可能作用。方法:采用激光共聚焦显微镜、多参数流式细胞技术对手术切除的21例喉鳞状细胞癌(7例伴颈淋巴结转移,14例不伴颈淋巴结转移)组织标本中Cyclin E的表达含量进行检测分析。结果:Cyclin E在喉鳞状细胞癌、阳性淋巴结、阴性淋巴结中均有不同程度的表达,喉鳞状细胞癌伴颈淋巴结转移平均荧光强度为78.12±6.54,略高于不伴颈淋巴结转移者(74.93±8.82)(P>0.05);阳性淋巴结平均荧光强度为93.65±7.48,明显高于阴性淋巴结(30.65±7.48)(P<0.01),且高于喉鳞状细胞癌伴颈淋巴结转移和不伴颈淋巴结转移者(P<0.05)。结论:Cyclin E的异常表达是喉癌发生、发展过程中一个重要的分子事件;Cyclin E的检测可望成为临床评估喉癌淋巴结转移的指标之一。
Objective：To study the expression of Cyclin E proteins in carcinogenesis and development of the metastasis of laryngeal squmous cell carcinomas （LSCC）. Method：The expression of Cyclin E protein were detected by laser scanning confocal microscopy （LSCM） and multiparameter flow eytometry （FCM） after immunofluorescence staining method in twenty-one surgically resected primary LSCC （7 LSCC with cervical lymph node metastasis, 14 LSCC without cervical lymph node metastasis）. Result： The fluoresence intensity was 78.12±6.54 in LSCC with cervical lymph node metastasis and 74. 93±8.82 in LSCC without cervical lymph node metastasis （ P 〉0. 05）; The fluoresence intensity in cervical lymph node metastasis was 93.65±7.48, it＇s more highter than that in cervical lymph node negative metastasis（ P〈0.01） and both LSCC with cervical lymph node metastasis and without cervical lymph node metastasis（ P〈0.05）. Conclusion： It is suggested that Cyclin E proteins may play an important role in the carcinogenesis and development of the lymph node metastases of LSCC. Detection of Cyclin E maybe becomes an indication to evaluating the lymph node metastases of LSCC.
出处
《临床耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2006年第6期262-263,267,共3页
Journal of Clinical Otorhinolaryngology
关键词
喉肿瘤
癌
鳞状细胞
淋巴转移
细胞周期素
E
Laryngeal neoplasms
Carcinoma, squamous cell
Lymphatic metastasis
Cyclin E
