摘要
目的 观察 ５／６肾切除大鼠肾脏细胞凋亡及凋亡相关基因 Ｂａｘ、Ｂｃｌ－２、Ｆａｓ和 Ｆａｓ－Ｌ的表达变化及其意义。方法 ＳＤ大鼠行５／６肾切除，术后６周、１２周采集标本，除常规光镜、电镜观察外．利用ＴＵＮＥＬ及流或细胞术，观察肾脏细胞凋亡及凋亡相关基因表达的变化，并结合肾功能变化分析肾脏细胞凋亡及凋亡相关基因表达与肾小球、肾小管及间质病变的关系。结果５／６肾切除大鼠肾脏细胞凋亡增加，凋亡相关基因表达增强，这些变化与肾脏形态和功能变化相平行 。结论 肾脏细胞凋亡及凋亡相关基因表达的变化可能参与肾小球硬化、肾小管萎缩及间质纤维化的病理过程。
Objective To investigate the role of apoptosis and expression of apoptosis-related genes in renal sclerosis. Methods Remnant kidneys were produced by 5/6 nephrectomy. The tissues of remnant kidneys were used to detect apoptosis and the cxpression of Bax, Del-2, Fas and Fas-L by TUNEL and flow cytometry. Results The number of apoptotic cells was more and the expression of Bax, Dcl-2, Fas and Fas-L was higher in the remnant kidneys. The successive changes of thase parameters were parallel to the morphological and functional alterations of the kidneys. Conclusion Apoptosis and the related genes may be involved in the development of chronic renal sclerosis.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2001年第5期309-311,共3页
Chinese Journal of Physical Medicine and Rehabilitation
基金
教育部归国博士基金资助项目
