摘要
本文利用细胞分光光度技术,系统地、多阶段地观察了大鼠肝癌发生过程中 DNA 含量的变化规律。结果显示3′-Me-DAB 诱癌两个月后,二倍体肝细胞就有明显的增加,在癌前病变中二倍体肝细胞进一步增加;早期肝癌及癌旁细胞是二倍体型的,提示缺乏分化能力的二倍体肝细胞在肝癌发生发展中有着重要的意义,它们很可能是细胞转化的一个重要标志。此外,癌细胞 DNA 含量结合病理形态更能客观地反映肿瘤恶性程度。
The nuclear DNA content of rat hepatocytes at different stages of hepatocarcinogenesis was measured on cytologic smears using microspe-ctrophotometer at wavelength of 560 nm. A significant reduction in the ratio of tetraploid to diploid cells was induced by oral admimistration of 0.06% 3'-methyl-4,4'-dimethylaminoazobenzene(3'-Me-DAB)for 2 months.Those rats treated with 0.06% 3'-Me-DAB for 7 months had a permanently de- pressed ratio of tetraploid:diploid.The tumor cells at the early stage of carcinogenesis and the percancerous hepatocytes were diploid in pattern.We suggest that the selective outgrowth of diploid cell population would significantly increase the likelihood of developing further to- wards cancer.It might be an important marker of cell transformation.The DNA content and pathomorphology of hepatoma might objectively reflect its malignity.
出处
《昆明医学院学报》
1991年第2期15-19,共5页
Journal of Kunming Medical College
关键词
肝肿瘤
甲基奶油黄
脱氧核糖核酸
Rat
Hepatocytes
DNA
3′-Methyl-4
4′-dimethy
aminoazobenzene
