摘要
目的 明确 5- Aza- cd R对 DNA MTase转录水平的抑制作用。方法 用 5× 1 0 -7M的5- AZa- cd R处理肝癌细胞株 SMMC- 772 1和 H2和 Hep G2 ,就用 RT- PCR技术检测经药物处理前后 2株肝癌细胞 DNA MTase m RNA表达水平。结果 经 5- Aza- cd R处理后 ,2株肝癌细胞 DNAMTase m RNA表达量显著下降。结论 5- Aza- cd R能抑制肝细胞 DNA MTase转录 。
Objective To under stant mechanism of inbhbition of DNA MTase by 5 Aza cdR.Methods detected the mRNA expression of DNA MTase gene by RT PCR in the hepatocellular carcinoma cell line SMMC 7721 and HepG 2 before and after treatment with 5 Aza cdR. Results In comparison with control group,mRNA expression are dramatically decreased at both of cell lines.Conclusion We concladed that 5 Aza cdR can inhibit the mRNA expression of DNA MTase.
出处
《肿瘤防治研究》
CAS
CSCD
2001年第4期294-295,共2页
Cancer Research on Prevention and Treatment
