摘要
从安徽、河南两地黄牛犊腹泻粪样中分离出了３株能在ＭＡ－１０４细胞上稳定繁殖、继代的轮状病毒（标号为ＢＲＶ００７、ＢＲＶ０１４及ＨＮ－７）。中和试验表明，该３株黄牛分离毒与北京奶牛毒ＢＲＶ６５５５及国外参考毒株ＮＣＤＶ之间抗原性无差异，同属轮状病毒血清６型（或牛轮状病毒血清１型），在亚组抗原特异性上均为第Ⅰ亚组。用ＢＲＶ０１４高代次（５０代以上）细胞培养物（ＴＣＩＤ５０＝１０－６．５）于孕母牛产前３个月和１个月经肌肉免疫注射２次，可明显提高母牛初乳中的轮状病毒抗体，在产后２０ｄ，抗体滴度仍能维持在１∶６４；临床观察，免疫母牛所产犊牛的腹泻发生率较对照组降低８３．８％。
Three strains (BRV007, BRV014 and HN 7) of bovine rotavirus were isolated in MA 104 from diarrheal calves in Anhui and Henan province. There were no differences among all 3 strains of local BRV and NCDV, as well as BRV6555 isolated in Beijing, so they were considered as serotype 6 (or bovine serotype 1) and subgroup Ⅰ. Vaccine were prepared from BRV014 over 50 passage in MA 104. Antibodies against rotavirus in colostrum from cows vaccinated twice intramuscularly with the vaccine increased obviously and prevented calves from the diarrhea efficiently.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1998年第3期224-227,共4页
Chinese Journal of Veterinary Science
