摘要
目的 :对石斛属植物亲缘关系进行分析 ,并设计一对能方便准确地鉴别铁皮石斛的特异性引物。方法 :用随机扩增多态性DNA(RAPD)技术对石斛属内 2 6个种和金石斛属的 1个种进行了基因组DNA多态性分析 ,并构建聚类树状图。根据筛选到的DNA片段序列 ,将Sangon18引物从 3’端延长至 2 0bp ,得到所要的特异性引物。结果和结论 :设计成的特异性引物能方便快捷地鉴别出铁皮石斛。此技术为药材的分子鉴定提供了一个新思路。
Objective: To analyze the genetic relationship of Dendrobium and design a specific primer to differentiate Dendrobium candidum effectively. Method: Random amplified polymorphic DNA (RAPD) technique was used to analyze the genetic relationships of 26 species of Dendrobium and one species of Ephemerantha. Dendrogram was constructed by UPGMA. According to the sequence of DNA fragment selected, Sangon 18 primer had been extended from 3' extreme to 20 bp in order to form a specific primer. Result and Conclusion: This primer can be used to distinguish Dendrobium candidum from other Dendrobium effectively. Such a technique provides a new way for the identification of Chinese traditional medicines. [
出处
《中国中药杂志》
CAS
CSCD
北大核心
2001年第7期442-447,共6页
China Journal of Chinese Materia Medica
