摘要
采用分子生物技术包括任意引物聚合酶链式反应（ＡＰＰＣＲ）和随意引物扩增的多态性ＤＮＡ（ＲＡＰＤ）方法扩增蒲公英及其６种土公英混淆品的基因组ＤＮＡ，获得清晰可靠的ＤＮＡ指纹图谱。根据琼脂糖凝胶上显示的ＤＮＡ带型差异可鉴别蒲公英和土公英混淆品。
DNA fingerprinting for Taraxacum mongolicum and its adulterants of six species of Compositae was demonstrated with random primed polymerase chain reaction (PCR) including arbitrarily primed PCR(AP PCR) and random amplified polymorphic DNA(RAPD).Distinctive genomic fingerprints from DNA of Taraxacum mongolicum and its adulterants were generated with two long (20 24 mer )and one short (10 mer) random chosen primers Taraxacum mongolicum can be distinguished from six adulterants according to the banding patterns of their amplified DNA on agarose gels. Moreover,that Taraxacum mongolicum and its adulterant are unrelated. The Similarity Indexes values of the genomic fingerprints.Results showed AP PCR and RAPD might be used for identifying Chinese drugs.
出处
《中国中药杂志》
CAS
CSCD
北大核心
1997年第4期197-200,共4页
China Journal of Chinese Materia Medica
基金
香港政府研究资助局资助
关键词
药材鉴定
蒲公英
聚合酶链式反应
DNA指纹图谱
Chinese drug
identification
Taraxacum mongolicum
polymerase chain reaction
DNA fingerprinting
