期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

重组BPI_(23)-Fcγ1融合蛋白在CHO细胞中的表达 被引量：6

Expression of Recombinant BPI_(23)-Fc γ1 Fusion Protein in CHO Cells
在线阅读 下载PDF
导出
摘要 The fusion gene of BPI 23 and human Fcγ1 was obtained by PCR method,and the expression plasmid was constructed to express recombinant BPI 23 \|Fcγ1 fusion protein in CHO cells.After transfection with the plasmid and selection by methotrexate,the cell lines expressing the fusion protein were obtained.The recombinant protein was purified using cation\|exchange chromatography and its bioactivity was proved with bactericidal assays. The fusion gene of BPI 23 and human Fcγ1 was obtained by PCR method,and the expression plasmid was constructed to express recombinant BPI 23 \|Fcγ1 fusion protein in CHO cells.After transfection with the plasmid and selection by methotrexate,the cell lines expressing the fusion protein were obtained.The recombinant protein was purified using cation\|exchange chromatography and its bioactivity was proved with bactericidal assays.
作者 徐俊杰 徐静 童贻刚 王海涛
机构地区 军事医学科学院微生物流行病研究所
出处 《生物工程学报》 CAS CSCD 北大核心 2001年第5期587-589,共3页 Chinese Journal of Biotechnology
关键词 杀菌/通透性增加蛋白 BPI23-Fcγ1融合蛋白 表达 CHO细胞 重组 bactericidal/permeability\|increasing protein, BPI 23 Fcγ1 fusion protein, expression, CHO cells
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献8

  • 1Elsbach P.The bactericidal permeability-increasing protein(BPI) in antibacterial host defense[].Journal of Leukocyte Biology.1998
  • 2Gray P W,Corcorran A E,Eddy R L et al.The genes for LBP and BPI are encoded in the same region of human chromosome 20[].Genomics.1993
  • 3Gazzano-Santoro H,Meszatos K,Birr C,et al.Competition between rBPI23 and rLBP to LPS and gram-negative bacteria[].Infection and Immunity.1994
  • 4Elsbach P,Weiss J.Role of the bactericidal permeability-increasing protein in host defenses[].Current Opinion in Immunology.1998
  • 5Elsbach P,Weiss J,Levy O et al.Preliminary evaluation of recombinant amino-terminal fragment of human bactericidal permeability-increasing protein in children with severe meningococcal sepsis[].The Lancet.1997
  • 6Gray P W,Flaggs G,Leong S R et al.Cloning of the cDNA of a human neutrophil bactericidal protein[].Journal of Biological Chemistry.1989
  • 7Beamer L J,Carroll S F,Eisenberg D.Crystal structure of human BPI and two bound phospholipids at 2. 4 angstrom resolution[].Science.1997
  • 8Http://www.xoma.com/ .

同被引文献59

  • 1李新生,高凤山,李云岗,崔保安,陈红英,崔沛,夏春.北京长白杂交猪IgGH链基因CH2-CH3的克隆、序列分析及表达质粒构建[J].河南农业大学学报,2006,40(1):1-6. 被引量：3
  • 2Chockalingam A, McKinney CE, Rinaldi M, et al. A peptide derived from human bactericidal/permeability-increasing protein (BPI)exerts bactericidal activity against Gram-negative bacterial isolates obtained from clinical cases of bovine mastitis [J]. Veterinary Microbilogy, 2007,125(1-2):80-90.
  • 3Chockalingam A, Zarlenga DS, Bannerman DD. Antimicrobial activity of bovine bactericidal permeability-increasing proteinderived peptides against gram-negative bacteria isolated from the milk of cows with clinical mastitis [J]. American journal of veterinary research, 2007,68(11): 1151-1159.
  • 4萨母布鲁,等,金冬雁,黎孟枫,等(译).H分子克隆实验指南(第二版)[M].北京:科学出版社,1996:765-780,888-887.
  • 5Weiss J, Elsbach P, Olsson I, et al.Purification and characterization of a potent bactericidal and membrane active protein from the granules of human polymorphonuclear leukocytes [J]. The Journal of Biological Chemistry, 1978,253:2664-2672.
  • 6Ooi CE,Weiss J,Doerfler ME, et al.Endotoxin-neutralizing properties of the 25kD N-terminal fragment and a newly isolated 30kD C-terminal fragment of the 55-60kD bactericidal/ permeability-increasing protein of human neutrophils[J].Journal of Experiment Medcine, 1991,174(3):649-655.
  • 7Gazzano-Santoro H, Parent J B, Grnna L,et al.High-affinity binding of the bactericidal/permeability-increasing protein and a recombinant amino-terminal fragment to the lipid A region of lipopolysaccharidd[J].Infection and Immunity, 1992,60 (11):4754-4761.
  • 8Horwitz AH,Ammons WS,Bauer RJ.rBPI(10-193)is secreted by CHO cells and retains the activity of rBPI21 [J]. Journal of Endotoxin Research,2004,10(2):97-106.
  • 9Schultz H,Weiss JP.The bactericidal/permeability-increasing protein (BPI)in infection and inflammatory disease [ J ]. Clinica Chimica Acta, 2007,384:12-23.
  • 10Levy O.A Neutrophil-Derived Anti-Infective molecule:Bacte- ricidal/Permeability-Increasing [J].Antimicrobial Agents and Chemotherapy, 2000,44(11):2925-2931.

引证文献6

二级引证文献7

生物工程学报
2001年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部