摘要
A gene library of the infectious spleen and kidney necrosis virus(ISKNV) from mandarinfish,Siniperca chuatsi(Basilewsky) genome was established ISKNV DNA was cleaved with EcoRI,BamHI,HindⅢ,KpnI and PstI and the resulting fragments were inserted into the corresponding sites of the pBluescript Ⅱ KS plasmid vector using T4 DNA ligase Bacterial colonies harboring recombinant plasmids were selected All cloned fragments were individually identified by digestion of the recombinant plasmid DNA with different restriction enzymes and screened by hybridization of recombinant plasmid DNA to viral DNA Using these recombinant plasmids,the physical maps of the genome were constructed for BamHI,HindⅢ and KpnI restriction
A gene library of the infectious spleen and kidney necrosis virus(ISKNV) from mandarinfish,Siniperca chuatsi(Basilewsky) genome was established ISKNV DNA was cleaved with EcoRI,BamHI,HindⅢ,KpnI and PstI and the resulting fragments were inserted into the corresponding sites of the pBluescript Ⅱ KS plasmid vector using T4 DNA ligase Bacterial colonies harboring recombinant plasmids were selected All cloned fragments were individually identified by digestion of the recombinant plasmid DNA with different restriction enzymes and screened by hybridization of recombinant plasmid DNA to viral DNA Using these recombinant plasmids,the physical maps of the genome were constructed for BamHI,HindⅢ and KpnI restriction endonucleases
出处
《病毒学报》
CAS
CSCD
北大核心
2001年第3期273-276,共4页
Chinese Journal of Virology
基金
广东省自然科学基金资助项目 (990 2 5 5 )
关键词
鳜鱼
传染性脾肾坏死病毒
基因组文库
物理图谱
infectious spleen and kidney necrosis virus(ISKNV)
molecular cloning
physical mapping
