摘要
鳜传染性脾肾坏死病毒（ＩＳＫＮＶ）是近年来危害广东地区鳜养殖业的主要病原。本文用回接感染健康鳜获得病毒原料，分离纯化了大量高纯度的ＩＳＫＮＶ病毒粒子，电镜下可见病毒粒子为二十面体，直径平均为１５０ｎｍ。抽提病毒核酸，对其基因组ＤＮＡ进行酶切分析，病毒基因组为双链ＤＮＡ分子，表现脊椎动物虹彩病毒基因组的特点即其胞嘧啶５’端高度甲基化。以限制性内切酶ＥｃｏＲＩ、ＢａｍＨＩ、ＨｉｎｄＩＩＩ、ＫｐｎＩ和ＰｓｔＩ酶切ＩＳＫＮＶ基因组ＤＮＡ，经电泳分离后，分别得到１、８、９、１８、２２条清晰的酶切片段，并根据酶切片段算得基因组的大小超过１０８．７ｋｂｐ。
Infectious spleen and kidney necrosis virus (ISKNV) from mandarinfish, Siniperca chuatsi (Basilewsky), a member of the Iridoviridae family, is the causative agent of a disease causing high mandarinfish mortalities and severe damage to mandarinfish cultures in China. The abundant virus material was obtained by experimental infection. Then ISKNV virions were isolated and purified. Virions are icosahedron, 150nm in diameter averagely as observed by transmission electron microscopy. The genome of ISKNV is a double-stranded DNA molecule and highly methylated at cytosines in the CpG. As the viral DNA was digested with EcoRI, BamHI, HindIII, KpnI and PstI restriction endonucleases and separated by electrophoresis, restriction fragments were clearly shown on the agarose gel. The size of the ISKNV DNA was estimated to be longer than 108. 7kbp.
出处
《水产学报》
CAS
CSCD
北大核心
2001年第3期238-243,共6页
Journal of Fisheries of China
基金
广东省自然科学基金资助项目(990255)
关键词
鳜鱼
传染性脾肾坏死病毒
纯化
酶切分析
Siniperca chuatsi
infectious spleen and kidney necrosis virus( ISKNV)
purification
enzyme analysis
