摘要
本文利用发根农杆菌感染大豆不同外植体,在成熟胚靠近子叶节部位诱导产生毛状根。经冠瘿碱检测表明,毛状根及由此产生的愈伤组织均有甘露碱存在,说明Ri质粒的T-DNA已整合到大豆的转化根及愈伤组织中。转化根再生实验表明,在含NAA和IAA8mg/L的MS培养基上得到不定根的分化,MS和B_3培养基及6％蔗糖对转化丛生芽的诱导有利。转化的丛生芽在MS基本培养基上进一步长成小植株。
Roots Were only induced at cotyledons node of mature embroyos in various explants of Glycine soja Glycine gracilis Glycine max with Agrobacterium rhizogenes. lurpection of opine showed that there was mannopine in the hairy roots induced with Agrobacterium rhizogenes and callus come from them and therefore that T-DNA of Ri-plasmid -was successfully transfered. The results of the experiment about regeneration of hairy roots showed that differentiation of adventitious root was only obtained in cuiture media supplementing 8mg/L NAA or IAA. It was found that kinds of basal medium MS and Bs with 6 % sucrose were effective in induction of multiple-buds. The multiple-buds of transformation have hrown plant on the basal medium MS.
出处
《生物技术》
CAS
CSCD
1991年第4期17-21,共5页
Biotechnology
关键词
发根农杆菌
毛状根
转化
大豆
Hairy root
Transformation
Glycine soja
Glycine gracilis
Glycine max
Agrobacterium rhizogenes
