摘要
以两性霉素B抗性作为标记 ,通过单株灭活 ,进行栖土曲霉种内非营养缺陷型原生质体融合 .在 40 %的PEG(Mr=6 0 0 0 )促融下 ,融合频率为 1.6 2× 10 -5.连续传接 10代后 ,获得稳定的融合子 .对孢子体积、核DNA含量、生长速度进行了测定 ,并利用RAPD技术分析比较了亲本及融合子的基因组DNA指纹多态性 ,证明融合子为杂合二倍体 ,产角蛋白酶活力较亲本显著提高 .图 3表 4参
Amphotericin B-resistant ST 1 and UV-inactivated Aspergillus terricola LT 2 protoplasts were fused in 40% PEG( M r=6 000) with a fusion rate of 1.62×10 -5 .And after 10 generations, the spore volumes, DNA contents and growth of the fusion bodres were measured, and the polymorphisms of genomic DNA fingerprints were compared among the recombinant and its two parents. The result showed it was hybrid. The keratinase production of the hybrid was greatly higher than that of the parents. Fig 3, Tab 4, Ref 17
出处
《应用与环境生物学报》
CAS
CSCD
2001年第4期388-391,共4页
Chinese Journal of Applied and Environmental Biology
基金
沈阳市青年科学基金资助项目
