摘要
以从自然界中筛选的新月弯孢菌Ｄ－１为出发菌株，进行原生质体制备及再生条件的研究。将培养至１６ｈ的Ｄ－１菌丝体经ＤＴＴ溶液处理３０ｍｉｎ后，用溶壁酶和纤维酶的混合酶液于３０℃下酶解４ｈ，原生质体释放量达到６．０×１０＾６个／ｍＬ，原生质体再生率为８．３％。
This Paper studies the conditions of preparation and regeneration of Cuvrularia lunata. The myceliawere cultured for 16h and suspended in 0.6mol/L KCI, after dealing by DTT for a short time, then Lywallzyme(1%) and Cellulase (1%) were added. The forming number of protoplasts was 6.0× 106 / mol and theregeneration frequency was 8.3%.
出处
《微生物学通报》
CAS
CSCD
北大核心
1999年第1期21-23,共3页
Microbiology China
基金
天津市科技攻关资助
关键词
新月弯孢霉
原生质体
制备
再生
Curvularia lunata, protoplasts, preparation, regeneration
