摘要
目的 探讨鼻咽癌中血管生长因子VEGF、bFGF的表达及其与颈淋巴结转移的关系。方法 采用免疫组化S -P法对 35例鼻咽癌 (其中 15例同时取颈部转移癌组织 ) ,2 0例鼻咽粘膜不典型增生组织和 10例慢性炎症组织中VEGF、bFGF的表达进行了检测。结果 ① 35例鼻咽癌中 17例 (4 8.6 % )VEGF表达阳性 ,明显高于非癌对照组 (6 .7% ,P =0 .0 0 0 ) ;有颈淋巴结转移鼻咽癌中VEGF的表达 (73.3% )明显高于无颈淋巴结转移者(30 % ,P =0 .0 18) ;晚期 (III、IV期 ,70 .6 % )明显高于早期 (Ⅰ、Ⅱ期 ,2 7.8% ,P =0 .0 18) ;但鼻咽癌原发灶中VEGF的表达与颈部转移灶中的表达无相关性 (P >0 .0 5 ) ;② 35例鼻咽癌中有 8例 (2 2 .9% )bFGF表达阳性 ,明显高于非癌对照组 (3.3% ,P =0 .0 31)。但鼻咽癌中bFGF的表达与患者的年龄、性别、肿瘤的组织学类型和临床分期无关 ,也与肿瘤的N分期无关 (P >0 .0 5 )。结论 ①VEGF与鼻咽癌的生长及颈淋巴结转移有关 ,它可能可以作为预测鼻咽癌颈淋巴结转移的指标 ;②bFGF可能与鼻咽癌的生长有关系 。
Objective To investigate the expression of vascular endothelial growth factor(VEGF), basic fibroblast growth factor (bFGF) and its possible role in cervical lymph node metastasis in nasopharyngeal carcinoma (NPC). Methods The expression of VEGF and bFGF was assessed by immunohistochemistry in 15 NPC with cervical lymph node metastasis, 20 NPC without lymph node metastasis or metastasis in other sites, 20 nasopharyngeal epithelial dysplasias and 10 nasopharyngitises. Results ① A significant difference was observed between the VEGF-positive rate in NPC ( 48.6% ) and in non-NPC controls ( 6.7% , P=0.000), although we could not find a relationship between the VEGF expression in primary lesions and in lymph node metastatic lesions. And a significant difference was also shown between the frequency of VEGF positivity in patients with cervical lymph node metastasis ( 73.3% ) and in those without lymph node metastasis (30%, P=0.018). ② The bFGF-positive rate in patients with NPC was significantly higher than that in those with non-NPC (P=0.031), but no correlation was found between bFGF expression and age, sex, tumor histologic type, N stage or tumor clinical stage. Conclusion VEGF expression may play an important role in the development of tumor and in predicting patients at risk of cervical lymph node metastasis from NPC. bFGF may also be involved in the growth of NPC but with no correlation with cervical lymph node metastasis in NPC.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2001年第2期91-94,T004,共5页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
广东省自然科学基金资助 (A970 0 78)
广东省医学科学技术研究基金资助 (A1997169
A1999190 )
