摘要
目的微波诱变法筛选遗传稳定的木聚糖酶高产球毛壳霉菌株。方法活化球毛壳霉菌种,制备孢子悬液,在不同功率条件下对其进行辐照处理60 s,选择致死率在80%左右的辐照功率,在此功率条件下对其进行不同时间的微波诱变,经初筛和复筛后,采用DNS法测定木聚糖酶活性,筛选木聚糖酶高产菌株,并分析其传代稳定性。结果在微波辐射时间80 s,功率240 W条件下处理的球毛壳霉孢子,致死率为89.5%;经诱变及菌种筛选,获得1株木聚糖酶高产菌株W80-8,其酶活力为7 520 U/ml,比出发菌株提高了124%;经6次传代培养,W80-8株木聚糖酶活力未见明显降低,未发生原位回复突变。结论成功筛选获得1株遗传稳定的木聚糖酶高产球毛壳霉菌株。
Objective To screen a high xylanase-producing Chaetomium globosum strain with genetic stability by microwave mutagenesis.Methods C.globosum strain was activated and prepared into spore suspension,then subjected to radiation at various powers for 60 s.The strain was subjected to microwave mutagenesis for various seconds at the selected radiation power with a lethal rate of about 80% and,after preliminary and repeat screenings,determined for xylanase activity by DNS method,based on which a high xylanase-producing strain was screened and analyzed for genetic stability.Results The lethal rate of spores of C.globosum was 89.5% after microwave radiation at a power of 240 W for 80 s.High xylanase-producing C.globosum W80-8 strain was obtained after mutagenesis and screening,of which the xylanase activity was 7 520 U / ml and increased by 124% as compared with that of original strain.Neither decrease of xylanase activity nor in situ back mutation was observed in W80-8 strain after subculture for 6 passages.Conclusion A high xylanase-producing C.globosum strain with genetic stability was successfully screened.
出处
《中国生物制品学杂志》
CAS
CSCD
2012年第3期375-377,共3页
Chinese Journal of Biologicals
基金
黑龙江省教育厅科学技术研究项目(11551324)
关键词
球毛壳霉
木聚糖酶
微波
诱变
Chaetomium globosum
Xylanase
Microwave
Mutagenesis
