摘要
酸性蛋白酶是酶制剂工业比较重要的酶种，广泛应用于饲料、食品、皮革、医药和酿造工业中［１～４］，具有较大的市场潜力。本研究以宇佐美曲霉（Ａｓｐｅｒｇｉｌｕｓｕｓａｍｉ）白色突变株Ｂ１为出发菌株，通过微波诱变和亚硝基胍、硫酸锂复合诱变剂点试平板法诱变，选...
A high yield acid protease producing strain Aspergrillus usamii L336 was screened from its parent strain B 1 by combining microwave irradiation with inducing of nitrosoguanidine and Li 2SO 4. By incubation of L336 in shaking flask at 31℃ for 88 h, its enzyme activity was 5500u/mL , and its properties for high yield producing acid protease remained stable after many times of subculture and storage for two months.
出处
《微生物学报》
CAS
CSCD
北大核心
1999年第2期181-184,共4页
Acta Microbiologica Sinica
基金
浙江省教育委员会资助
关键词
微波诱变
化学诱变
宇佐美曲霉
酸性蛋白酶
Microwave irradiation, Chemical inducing, Aspergrillus usamii L336, Acid protease
