摘要
目的 探讨大鼠急性水肿性胰腺炎 (AEP)动物模型的早期微循环改变及中药复方清下 1号 (MCP 1)对AEP胰腺微循环的作用。方法 用异硫氰酸荧光素 荧光标记红细胞 (FITC RBC)胰腺活体微循环技术、微血管树脂 /墨汁灌注光镜和扫描电镜、透射电镜技术 ,用蛙皮缩胆囊肽诱发大鼠急性胰腺炎 (AP)动物模型 ,观察 36只Wistar大鼠的早期微循环改变、MCP 1应用后胰腺局部微循环的反应。结果 与AEP自然病程组比较 ,MCP 1治疗组血清淀粉酶由 ( 2 997.7±80 1.4)IU/L降至 ( 190 9.7± 2 95 .5 )IU /L(P <0 .0 1) ;胰腺间质炎性细胞浸润减少 ;腺泡细胞胞浆内空泡减少 ;毛细血管密度由 ( 5 2 .8± 6.1) %增至 ( 63.2± 5 .5 ) % (P <0 .0 1) ;微血管管径由( 4.5± 0 .4) μm增至 ( 5 .9± 0 .6) μm ( P <0 .0 5 ) ;FITC RBC显示胰腺微循环流速、流量增加 ,灌流稳定 ( 0 .87± 0 .0 6)nl/min( P <0 .0 1)。结论 MCP 1具有显著改善AP胰腺微循环的作用 ,抗AP胰腺局部微循环损伤是实现MCP 1疗效的重要机制。
Objective To study the changes of the pan creatic microcirculation in caerulein-induced experimental acute pancreatitis a nd the effects of Chinese medicine No.1 (MCP-1) on the pancreatic microcircul ation of acute edematous pancreatitis (AEP). Methods Thir ty-six male wistar rats were studied by scanning electron microscopy of vascula r corrosion casts, transmission electron microscopy and light microscopy of Chine se ink-injected/cleared tissues,intravital fluorecence microscopy of erythrocy tes labeled by FITC (FITC-RBC). Results Compared with t he AEP group, animals in the CMP-1 treated group showed reduction of amylases [(2997.7±801.4) IU/L vs (1909.7±295.5) IU/L, P<0.01],reduction of q uantities of inflammatory cells infiltrated in the interstitial, reduction of qu antities of vacuoles in the acinar cells, an increase of the microvessel diamete r [(5.9±0.6) μm,P< 0.05] and the density of capillaries [(63.2±5.5)%, P<0.01)],and an increase of pancreatic blood flow/perfusion [(0.45±0.12) nl/min vs (0.87±0.06) nl/min, P<0. 01]. Conclusion Animals in the AEP gro up treated with MCP-1 showed significant changes in the early stage of acute pa ncreatitis in the pancreatic microcirculation. MCP-1 can improve the pancreatic microcirculation effectively.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2001年第4期298-300,F003,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目 (39770 72 2
3992 50 32 )
