摘要
目的 观察叶酸缺乏饮食对大鼠血浆同型半胱氨酸 (Hcy)水平及动脉组织的影响。 方法 雄性Wistar大鼠 2 0只均分成 2组 ,分别给予叶酸缺乏 (叶酸缺乏组 )和叶酸正常 (对照组 )饲料 ,饲养 3个月后检测血清叶酸和血浆Hcy浓度及红细胞超氧化歧化酶 (SOD)和谷胱甘肽过氧化物酶 (GPX)活力 ,实验后光镜检查主动脉组织学变化。 结果 叶酸缺乏组实验后血清叶酸浓度〔(6 0 8± 1 84) μg/L〕明显低于实验前〔(13 32± 2 0 2 ) μg/L〕和对照组〔(12 17± 1 6 7) μg/L〕 ,而血浆Hcy浓度〔(2 8 6 6± 6 0 7) μmol/L〕明显高于实验前〔(9 75± 1 86 ) μmol/L〕和对照组〔(9 49± 1 77) μmol/L ,P <0 0 1〕 ;红细胞SOD〔(37389 5± 5 15 8 4)NU/g·Hb〕高于对照组〔(30 35 5 7± 6 349 2 )NU/g·Hb〕 ,GPX活力〔(10 94± 3 0 5 )U/L〕低于对照组〔(17 93± 3 0 5 )U/L ,P <0 0 5〕 ;主动脉组织出现内皮细胞肿胀、变性、脱落 ,裸露的内膜附有血栓和胶原纤维 ,内皮下组织疏松并出现平滑肌细胞 ,内弹力膜断裂 ,中膜明显增厚。 结论 叶酸缺乏饮食可引发高Hcy血症和动脉损伤 。
Objective To observe effects of the folic acid deficient diet on plasma homocysteine(Hcy) level and lesions of aorta in rats. Methods Twenty male Wistar rats were divided into two groups, folic acid deficent group (FD) and control group (Ctl).The rats were fed with folic acid deficient diet and normal diet for 3 months,respectively. The levels of serum folic acid and plasma Hcy as well as the activities of superoxide dismutase(SOD) and glutathione peroxidase (GPX) in erythrocytes were measured.The histological changes of aorta were also examined by light microscope. Results After 3 months treated with folic acid deficient diet, serum folic acid level 〔(6 08±1 84)μg/L〕 decreased significantly in rats of FD group compared either with pre experimental level 〔(13 32±2 02)μg/L〕 or with control one 〔(12 17±1 67)μg/L〕. Meanwhile, plasma homocycteine level increased significantly in rats of FD group 〔(28 66±6 07)μmol/L〕 compared either with pre experimental level 〔(9 75±1 86)μmol/L〕 or with control one 〔(9 49±1 77)μmol/L〕. The activity of erythrocytic SOD increased but GPX decreased obviously. The morphology lesions were also observed in aortic tissue. Conclusions Folic acid deficient diet induced hyperhomocysteinemia and arterial lesions.The high oxidant stress induced by hyper homocysteinemia may be one of mechanisms of arterial lesion.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2001年第4期279-282,共4页
Chinese Journal of Geriatrics
