摘要
目的探讨不同剂量大豆异黄酮单独干预、大豆异黄酮与叶酸联合干预对神经胶质细胞损伤的保护作用,并对其可能的作用机制进行探讨。方法选用24 h龄ICR小鼠,以肝微粒体酶混合物(S9)诱导环磷酰胺制备神经胶质细胞的损伤模型,采用单细胞凝胶电泳技术(彗星试验)和MTT细胞活性试验,观察大豆异黄酮、叶酸及两者联合作用后对神经胶质细胞损伤的拮抗作用。结果彗星试验中,与模型组比较,各干预组神经胶质细胞的彗星细胞拖尾率(P<0.05)和尾长值均明显降低(P<0.01);随着大豆异黄酮浓度的增加,大豆异黄酮单独和联合干预组的彗星细胞拖尾率和尾长值逐渐降低,但两者的差异无统计学意义(P=0.052)。MTT试验中,与模型组相比,各干预组的细胞活性明显增强(P<0.05),随着大豆异黄酮浓度的增加,大豆异黄酮单独或联合干预组的OD492值逐渐升高,尤其是大豆异黄酮和叶酸联合干预比相应的大豆异黄酮单独干预效果明显(P<0.05)。结论大豆异黄酮可以在一定程度上抑制神经胶质细胞损伤和增强细胞的活力,两者联合作用的效果更佳,且存在一定的剂量-反应关系。提示大豆异黄酮和叶酸对神经胶质细胞具有较好的协同作用。
Objective To study the protective effects of soybean isoflavone and soybean isoflavone ooordinated with folic acid on cultured glial cells damaged by cyclophosphamide. Methods Cultured glial cells from new born ICR mice were exposed to cyclophosphamide in the presence of S9, a metabolic activator of cyclophosphamide. In order to evaluate the protective effectiveness of soybean isoflavone, folic acid and their combinative effect, the single-cell gel electrophoresis（SCGE, comet assay） and MTT assay were performed. Results Comparing to model group, the tail length of nuclear DNA and the percentage of comet-like tail glial cells were significantly reduced in solo-soybean isoflavone, solo-folic acid and soybean isoflavone ooordinated with folic acid group, and the reducing effects of co-administration were more efficiency than solo-administration, but not significantly（P = 0. 052）. Comparing to model group, the activity of glial cells was significantly increased in solo-soybean isoflavone, solo-folic acid and soybean isoflavone coordinated with folic acid group. Especially, the glial cell activity of co-administration group was significantly increased comparing to solo-administration （ P 〈 0. 05）. Conclusion Soybean isoflavone and folic acid are the protective factors of nervous system, and the protective action of co-administration would he more efficiently than soloadministration and the protective effects were dose-dependent.
出处
《首都医科大学学报》
CAS
2005年第6期673-676,共4页
Journal of Capital Medical University
基金
北京市教委基金启动资助项目
