摘要
为探讨大鼠幽门括约肌在化学性炎性痛时氮能和胆碱能神经元的变化 ,采用还原型辅酶 和亚铁氰化铜直接显色的组织化学法 ,分别显示一氧化氮合酶 (NOS)和乙酰胆碱酯酶 (ACh E)阳性神经元成分及甲醛诱发胃痛后 2种神经元成分的变化。结果显示 :甲醛致胃炎性痛时大鼠幽门括约肌粘膜下丛及肌间神经丛 NOS和 ACh E阳性神经成分活性均降低 ,仅于括约肌肠侧端或胃侧端偶见单个分布的 2种酶弱阳性胞体 ,肌内 2种神经纤维数减少 ,酶活性也弱。图像分析肌内 NOS和 ACh E阳性神经纤维活性平均光密度 ,甲醛组比对照组均显著降低 (P<0 .0 1)。提示在甲醛引起的胃急性化学性炎性痛时肠神经系统中 NO-和
The changes of nitronergic, cholinergic neurons in pyloric sphincter of the rats with gastric chemical inflammatory pain were investigated. NOS, AChE positive neurons in cryostat section of gastric sphincter were studied by NADPH and Karnovsky Roots histochemical methods after treated with formaldehyde. The results were as follows: There was no obvious transition form from gastric mucosa to intestine mucosa in pyloric sphincter. There were NOS and AChE positive neurons and fibers in Meissner's submucosal plexus (MSP) and Auerbach's myenteric plexus (AMP) of pyloric sphincter. In the intestine side, NOS positive cell bodies and fibers were more than those on the gaster side. There were AChE positive fiber near base membrane, and gastric side>intestine>middle in density. In AChE positive ganglia of gastric sphincter, there were many AChE positive cell bodies. There were ten cells in a plexus. Enzyme activity was intensity. The activity of NOS /AChE in MSP and AMP of pyloric sphincter was decreased with formaldehyde induced chemical inflammatory pain. It was suggested that NO /ACh might affect constraction and relaxion of sphincter directly and regulate function of gastrointestinal tract with the inflammatory pain.
出处
《同济医科大学学报》
CAS
CSCD
北大核心
2001年第4期307-309,F003,共4页
Acta Universitatis Medicinae Tongji
基金
卫生部科研基金资助项目 (No.96 - 1- 139)
关键词
一氧化氮合酶
乙酰胆酯酶
肠神经系统
大鼠幽门括约肌
化学性炎性胃痛
nitric oxide synthase
acetylcholinesterase
enteric nervous system
pyloric sphincter
chemical inflammatory pain
rat
