摘要
目的：一氧化氮（ＮＯ）是一种新发现的胃肠道抑制性神经递质，本组探讨ＮＯ在肠动力性疾病的发病机制中的意义。材料和方法：应用尼克酰胺腺嘌呤二核苷酸磷酸纳盐（ＮＡＤＰＨ）黄递酶、乙酰胆碱脂酶（ＡｃｈＥ）组织化学技术和Ｐ物质（ＳＰ）免疫组化技术观察７例先天性巨结肠（ＨＤ）、１４例便秘及３４例肠易激综合征（ＩＢＳ）病人结肠壁内ＮＯＳ、ＡｃｈＥ及ＳＰ阳性神经的分布状况，并与１３例“正常”结肠组织对照。结果：ＨＤ病人无神经节细胞肠段内含一氧化氮合成酶（ＮＯＳ）和ＡｃｈＥ的神经元完全缺失，胆碱能节前纤维异常增生，而有神经节细胞肠段呈正常表现；便秘病人结肠壁内ＮＯＳ阳性纤维比对照明显增多，而ＳＰ阳性纤维则显著减少；ＩＢＳ便秘型肠壁内ＮＯ能神经成分增多，腹泻型者减少。结论：肠ＮＯ能神经的紊乱可能与肠动力性疾病的发生有一定关系。
Objective: Nitric oxide(NO) has resently been identified as an inhibitory neurotransmitter in human colon. Methods: NADPH-diaphorase and AchE histochemistry and SP immunohisto-chemistry assays were used to study the distribution of NOS、AchE and SP in the colonic tissue from Hirschsprung's disease(HD) . obstipation and irritable bowel syndrome (IBS) and normal controls. Results: In the aganglionic segment in HD, NO synthase activity was almost completely absent and AchE-positive nerve fibers were apparently increased in the plexus area and in the musculature. In contrast, in the ganglionic segment, NO synthase and AchE were abundant, similar to normal NO-containing nerves were richer in constipative patient but lesser is SP as compared with normal controls, NO-containing nerves were also more abundant in constipation predominent IBS than in diarrhea predominant IBS, but NO synthase activity in the vessels of the latter was higher than that in control. Conclusion: These results suggest that NO may play a role in the intestinal motility disorders.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1997年第4期195-198,共4页
Chinese Journal of Digestion
基金
卫生部科学基金
关键词
一氧化氮合成酶
先天性
巨结肠
便秘
Nitric oxide synthase NADPH-diaphorase Hirschsprung's disease Constipition Irritable bowel syndrome
