摘要
目的通过门静脉输注同种异基因脾细胞、腹腔给予环磷酰胺诱导同种心脏移植耐受 ,并探讨其耐受机理。方法经门静脉给予受体大鼠 3× 10 8个异基因供体脾细胞 ,2 d后腹腔注射 80 m g/kg的环磷酰胺 ,10 d后实施心脏移植手术。观察、记录移植物的存活时间 ,通过 ML R、DTH、IL- 2逆转实验及过继性转移实验 ,探讨耐受机理。结果异基因心脏移植物的存活时间明显延长 ;ML R和 DTH实验证明 :受体大鼠的免疫应答受抑制 ,且该耐受表现为供体特异性 ;IL- 2逆转实验、过继性转移实验表明 :该耐受与克隆失活、抑制细胞和“感染”耐受机理有关。结论本诱导方案对于大鼠心脏移植存活时间的延长是一有效的方法 ,移植耐受的形成涉及多种机制。
ObjectiveTo induce cardiac transplantation tolerance by injecting spleen cells through portal vein, and then cyclophosphamide(CP)administration by intraperitoneal route to study the mechanism of transplantation tolerance. Methods Inject 3×10 8 allogeneic spleen cells to recipient rats by portal vein. Cyclophosphamide was injected intraperitoneally in adose of 80 mg/kg 2 days later.Heart grafting was carried out on day 10 after CP treatment.The survival time was observed and monitored.The tolerant mechanism was investigated by MLR,DTH,IL-2 reverse and adoptive transfer test. ResultsCardiac allograft survival was significantly prolonged; MLR and DTH demonstrated that immune response of recipient rats was inhi- bited and specific for donors. The IL-2 reverse and adoptive transfer showed that the tolerance mechanisms involve in anergy, suppressor cells and 'infectious' tolerance. ConclusionsThe tolerance protocol is an effect method for prolongation of rats cardiac allograft survival.The transplantation tolerance may include several mechanisms.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2001年第4期247-250,共4页
Immunological Journal
基金
国家自然科学基金重点项目 ( 3 983 0 3 4 0 )
关键词
移植耐受
脾细胞
环磷酰胺
心脏移植
门静脉输注
大鼠
transplantation tolerance
spleen cells
cyclophosphamide
cardiac transplantation
