摘要
目的 :探讨学习记忆的形成和遗忘与突触可塑性的关系。方法 :用水迷宫训练大鼠 2 1天建立空间辨别性学习记忆模型 ,随后停止训练 ,时间分别为 3天、7天及 1 4天 ,用免疫组化方法和图像分析技术 ,检测了突触素在大鼠海马CA3区表达的变化。结果 :对照组大鼠海马CA3区突触素的表达弱 ,免疫反应产物呈点状颗粒 ,沿辐射层长轴分布 ,多形层也有散在分布 ;模型组大鼠突触素染色明显比对照组的深 ,密集的小颗粒呈带状沿辐射层排列 ,在辐射层的底部可见一些大的阳性颗粒 ,随着停止训练时间的延长 ,突触素的表达逐渐减弱。结论 :空间学习记忆导致海马CA3区辐射层新突触的形成 ,而随着空间学习记忆的遗忘 ,又致新形成突触的消退。
To study the relationship between the synaptic plasticity and the establishment and dementia during spacial learning and memory.Methods:In the model group,rats trained 21 days by water maze and stopped the training for 3,7 and 14 days.Immunohistochemical technique and computer-image analysis were used to study the change of synaptophysin in hippocampal CA3 field.Results:Expression of synaptophysin in CA3 was weak in control group,the immunoreaction product demonstrated dot granules distributed along the radiatum of CA3,and diffusely in the oriens.The stained granules were darker in the model group than in the control group,the density of granules was also higher than in the control group.Some large stained granules were found in the fundus of radiatum.Expression of synaptophysin in CA3 gradually decreased when the training time stopped.Conclusion:New synapses in radiatum of hippocampal CA3 field are formed during spacial learning and memory and disappear with dementia.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2001年第4期336-339,共4页
Chinese Journal of Anatomy
