摘要
建立了胶束电动毛细管色谱法分离和测定几种大黄中大黄素、芦荟大黄素、大黄酸含量的测定方法。缓冲液为含０．０２５ｍｏｌ·Ｌ－１十二烷基磺酸钠（简称ＳＤＳ）的０．０２５ｍｏｌ，Ｌ－１３－环己氨基－１－丙烷磺酸（简称ＣＡＰＳ）－乙腈（１００：１０），ｐＨ０．９６。线性范围：芦荟大黄素为０．００２５～０．０１７５ｍｇ·ｍｌ－１（ｒ＝０．９９９９），大黄素为０．００５～０．０５ｍｇ·ｍｌ－１（ｒ＝０．９９９９），大黄酸为０．００５～０．０３５ｍｇ·ｍｌ－１（ｒ＝０．９９９６）。回收率：芦荟大黄素为９８．２％～１０１．８％，大黄素９９．９％～１０１．９％，大黄酸９９．２／～１０１．２％。此法简便、快速、重现性好。
A micellar electrokinetic
capillary chromatographic(MECC) method for the separation and
determination of emodin, aloe-emodin and rhein in several Rheum
species was estab-lished. The buffer solution used in this method was
a mixture of 0.025 mol·L-1 3-cyclohexylamino-1-propane-sulfonic
acid(containing 0.025 mol·L-1 sodium dodecyl
sulfate)-acetonitrile(100:10 ) at pH 10. 9 The linear calibration
range was 2.5～17.5μg,ml-1(r=0.9999) for aloe-emodin,0～50μg·
ml-1(r=0.9999) for emodin and 5.0～35μg·ml-1(r=0.9996)for rhein.
The recovery was 98.2%～101.8%for aloe-emodin, 99.9%～101.9%for
emodin and 99.2%～101,2%for rhein. This method is simple, rapid and
reproducible.
出处
《药学学报》
CAS
CSCD
北大核心
1995年第8期594-598,共5页
Acta Pharmaceutica Sinica
关键词
大黄
色谱法
大黄素
芦荟大黄素
大黄酸
分离
Rheum
Micellar electrokinetic capillary
chromatography
Emodin
Aloe-emodin
Rhein
