摘要
为了探讨针灸对脑老化的作用机理 ,我们观察了针灸“百会”、“大椎”、“命门”穴对D 半乳糖诱导的亚急性衰老小鼠脑组织中一氧化氮 (NO)、丙二醛 (MDA)的含量和超氧化物歧化酶(SOD)的活性变化的影响。结果表明亚急性衰老小鼠脑组织中NO、MDA含量明显增高 ,SOD活性明显降低 ,针灸能使上述指标逆转。提示针灸“百会”、“命门”、“大椎”具有抗脑老化的作用 ,其作用机理可能与其能抑制自由基反应 。
Objective: To explore the mechanism of acupuncture in anti aging effect. Methods: 30 mice were randomly and evenly divided into control, model and model+acupuncture groups. Sub acute aging model was established by subcutaneous injection of D galactose (0.5 mL/L). “Baihui” (GV 20) was punctured; “Dazhui” (GV 14) and “Mingmen” (GV 4) were moxibustioned for 2×3 cones. After homogenating the brain tissue, nitric oxide (NO) content was detected with spectrophotometry, malonyldialdehyde (MDA) content assayed with thiobarbituric acid (TBA) method and superoxide didismutase (SOD) activity determined with xanthine oxidimetry. Results: In the brain tissues of the aging mice (model group), NO and MDA contents increased remarkably while SOD activity was lowered apparently compared with those of control group (P<0.01). After acupuncture treatment, the increased contents of NO and MDA were lowered significantly while the decreased SOD activity raised considerably in comparison with those of model group (P<0.01) but had no marked difference with control group (P> 0.05). Conclusion: Acupuncture has an effect in resisting cerebral aging by way of inhibiting reactions of free radical and raising the activity of antioxidase.
出处
《针刺研究》
CAS
CSCD
2001年第2期111-113,共3页
Acupuncture Research
