摘要
目的:观察缺氧缺血新生大鼠经过氦氖激光穴位照射后脑组织中的活性物质超氧化物歧化酶活性及丙二醛含量的变化情况。方法:实验于2003-03/11在郑州大学基础医学院人体解剖学教研室完成。选择30只7d龄健康Wistar大鼠,体质量12～15g,雌雄不拘。随机分为3组,即假手术对照组、缺氧缺血组和激光穴位照射组,每组10只。缺氧缺血组与激光穴位照射组动物均制作成缺氧缺血模型,在清醒状态下实施左侧颈总动脉结扎术,术后在室温中恢复1～4h后,置于常压缺氧仓中,通入8mL/L氧气、920mL/L氮气(体积比)的混合气体,2h后将动物取出。缺氧缺血组不加任何治疗。激光穴位照射组于缺氧缺血后第2天,采用氦氖激光照射百会、大椎两穴,功率4mW,光斑直径3mm,每次照射10min,1次/d,同一时间进行,10d为1个疗程,共3个疗程,各疗程间隔2d。百会穴在颅顶正中,大椎穴在C7～T1之间,背部正中。假手术组仅分离左侧颈总动脉,不结扎,不缺氧。各组大鼠常规饲养34d后,黄嘌呤氧化酶法测定超氧化物歧化酶活性,比色法测定丙二醛含量。结果:因缺氧缺血对动物造成了伤害,缺氧缺血组死亡3只,激光穴位照射组死亡2只。进入结果分析,假手术对照组、缺氧缺血组和激光穴位照射组分别为10,7和8只。①大鼠脑组织超氧化物歧化酶活性:缺氧缺血组显著低于假手术对照组和激光穴位照射组[(59.70±4.03)NU/mL,(76.24±2.43)NU/mL,(72.56±5.60)NU/mL,(t=5.03,4.83,P<0.05)]。②大鼠脑组织丙二醛含量:缺氧缺血组显著高于假手术对照组和激光穴位照射组[(267.58±30.55)mmol/L,(99.87±15.48)mmol/L,(135.29±32.76)mmol/L,(t=10.68,8.81,P<0.05)]。结论:氦氖激光穴位照射能提高、诱发缺氧缺血大鼠脑组织中超氧化物歧化酶的生成和活力,降低丙二醛水平。新生大鼠未成熟脑的可塑性较大,如果利用此关键时期进行氦氖激光穴位照射,可能对脑功能的恢复有一定帮助。
AIM: To observe the changes of superoxide dismulase activity and malondiadehyde content of brain constitution after He-Ne laser acupuncture point irradiation in neonatal rats with cerebral hypoxia ischemia. METHODS: The experiment was done in Department of Human Anatomy of Collage of Basic Medical Sciences of Zhengzhou University between March and November 2003. Thirty healthy Wistar rats at the age of 7 days with 12-15 g body mass, no matter male or female, were randomly divided into three groups with 10 rats in each group: sham operation control group; hypoxia ischemia group (H-I group); laser acupuncture point irradiation group. The rats in H-I group and laser acupuncture point irradiation group were made into hypoxia and ischemia model, and treated with ligation of common carotid artery under clearmindedness situation. They were put into hypoxia granary with normal pressure after resuming 1 to 4 hours under room temperature post-operation. Filling with mixture gas of 8 mL/L dephlogisticated air and 920 mL/L nitrogen fumes (volum ratio), 2 hours later the rats were took out. The rats in H-I group did not take part in any operation. The rats in laser acupuncture point irradiation group 2 days after hypoxia and ischemia were treated with Helium and Heon laser to irradiate Paihui (Du 20) and Tachui (Du 14), with 4 mW power, and 3 mm light spot diameter and 10 minutes every time and once per day at the same time and 10 days for 1 course, totally for 3 courses with every 3 days one course. Paihui was in the middle of calveria, and Tachui in the middle of back between C7-T1. The rats in sham-operated group only treated with the detachment on left-side common carotid artery without deligation, hypoxic. Rats in every group after 34 days routine breeding, the activity of superoxide dismutase (SOD) tested by xanthine oxidase and the content of malondialdehydein (MDA) was examined. RESULTS: Because hypoxia and ischemia cause injury on animals, 3 rats in H-I group died, and 2 rats in laser acupuncture point irradiation group died. Ten, seven and eight in sham operation control group, H-I group, and laser acupuncture point irradiation group were involved in results analysis respectively. ① The activity of SOD: It was significantly lower in H-I group than that in sham operation control group and laser acupuncture point irradiation group [(59.70±4.03) NU/mL, (76.24±2.43) NU/mL, (72.56±5.60) NU/mL,(t=5.03,4.83, P < 0.05)]. ② The content of MDA of brain constitution in rats: It was significantly higher in H-I group than that in sham operation control group and laser acupuncture point irradiation group [(267.58±30.55) mmol/L,(99.87±15.48) mmol/L,(135.29±32.76)mmol/L,(t=10.68,8.81, P < 0.05)]. CONCLUSION: He-Ne laser acupuncture point irradiation has significant positive effects on the increase of SOD activity and the decrease of MDA content in rat brain tissue after H-I. The compliance of immaturity brain in neonatal rats is huge. If the key period is applied to proceed He-Ne laser acupuncture point irradiation, which maybe have certain help on the recovery of brain function.
出处
《中国临床康复》
CAS
CSCD
北大核心
2005年第23期196-197,共2页
Chinese Journal of Clinical Rehabilitation
基金
河南省科技攻关项目(0211043800)~~
