摘要
目的 :观察神经生长因子 (NGF)基因在生后不同时期大鼠心肌组织中的表达。方法 :采用 RT- PCR方法 ,半定量分析生后 1天、15天、30天、6 0天、12 0天、36 0天大鼠心肌组织中 NGF m RNA含量。结果 :大鼠生后 1天 ,心肌组织即有 NGF m RNA的表达 ;随着生后发育过程 ,表达量逐步增加 ,30天时达峰值 ;此后随鼠龄的增加 ,表达量呈缓慢下降趋势。结论 :大鼠心肌组织中 NGF m RNA的表达量 ,随鼠龄的增加 。
Objective:To study NGF gene expression in the myocardium of rat during postnatal various period. Methods: NGF mRNA expression levels in the myocardium of rat 1d, 15d, 30d, 60d, 120d, 360d after born were investigated by the RT-PCR method, as a hamiquantity assay. Results: NGF mRNA in rat myocardium appeared in newborn. During the developmental period, NGF mRNA expression was gradually increased, and it reached the peak at 30th day after born. Then it slowly decreased following the extension of age. Conclusion :The expression levels of NGF mRNA in rat myocardium was possessed of the changeable character during postnatal various period, that was increased first and decreased then.
出处
《南通医学院学报》
2001年第3期224-225,228,共3页
ACTA Academiae Medicinae Nantong
基金
国家自然科学基金资助项目 ( No.30 0 70 2 55)
江苏省教育厅科研项目 ( OOKJD30 10 0 0 7)
