摘要
地高辛素标记人神经生长因子ＤＮＡ探针的制备及应用曹铮，陆璐，丁斐，顾静，刘飞，殷冬梅，顾晓松江苏南通医学院分子生物学研究室南通２２６００１余云开，汪家政北京军事医学科学院基础医学研究所北京１００８５０ＰＵＲＩＦＩＣＡＴＩＯＮＡＮＤＩＴＳＡＰＰＬＩＣＡ...
Recombinant human-βNGF DNA/pUC 19 was digested with Hind Ⅲ and Bam HI and h-βNGF DNA fragments were further purified by low-melting agarose electrophoresis. H-βNGF DNA were digoxigenin labeled with random priming. It could detect even 0.1 pg/μl h-βNGF DNA/pUC 19 in dot hybridization.In situ hybridization of the submandibular glands also showed the high specificity and sensitivity of this probe in measuring β-NGF mRNA.
出处
《神经科学》
SCIE
CAS
1995年第3期141-144,140,共4页
Chinese Journal of Neuroscience
