摘要
目的 :了解乙酰胆碱 (acetylcholine,Ach)对人垂体腺瘤细胞增殖的影响。方法 :将Ach作用于体外培养的人垂体腺瘤细胞 ,测定MTT反应A值和3H TdR参入量及用流式细胞仪测定细胞周期。结果 :10 -7～ 10 -5mol/LAch可剂量依赖性地使MTT反应A值和3H TdR参入量降低 ,使垂体腺瘤G1期细胞比例增加 (P <0 .0 1) ,并可被阿托品阻断。结论 :Ach在体外能明显抑制培养的人垂体腺瘤细胞的增殖 。
Aim:To study the influence of acetylcholine on the proliferation,DNA synthesis and cell cycle of cultured human pituitary tumour cells.Methods:MTT method, 3 H TdR incoporation and cell cycle analysis were used to examine the changes of proliferation and DNA synthesis of human pituitary tumour cells.Results:Ach at 10 -7 mol/L～10 -5 mol/L could decrease the 3 H TdR incoporation and the MTT A value in a dose dependent manner ( P <0.01),and the ratio of G1 phase of pituitary tumour cells increased markedly ( P < 0.01 ).The effect of acetylcholine on the proliferation of cultured human pituitary tumour cells could be inhibited by atropine.Conclusion:Ach inhibited the proliferation,DNA synthesis and cell cycle of cultured human pituitary tumour cells,and the inhibitory effect was mediated by acetylcholine receptor.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2001年第2期129-132,共4页
Chinese Journal of Applied Physiology
关键词
乙酰胆碱
垂体腺瘤
细胞增殖
acetylcholine
pituitary tumor cells
proliferation
