摘要
作者研究了柔红霉素(DNR)在低浓度(1～40 ng/ml)下对人早幼粒白血病细胞系(HL-60)细胞增殖和分化的作用。通过分析活细胞计数、[~3H]TdR和[~3H]UR参入、克隆形成试验、细胞形态以及NBT还原试验等各项指标,结果表明:1 ng/ml DNR即可对HL-60细胞克隆形成有抑制作用,未见促分化效应;大于5 ng/ml DNR有明显细胞毒效应,亦可促使分化细胞百分率增高,但分化细胞绝对数无明显增加。提示:小剂量DNR的主要作用机理仍在于抑制细胞增殖和细胞毒效应,诱导分化作用并不显著。
In order to probe into the mechanism of low-dose chemotherapeutic agents in the treatment of leukemia, we studied the effects of low concentrations (l-40ng/ml) of daunorubicin (DNR) on proliferation and differentiation of human promyelocytic leukemia cell line (HL-60). 1 ng/ml DNR could inhibit the proliferation of the cells, but no induction of differentiation was found. DNR at more than 5 ng/ml had both cytotoxicity and induction of differentiation. Through the count of survival cells, [3H]TdR and [3H]UR incorporation, cloning efficiency, cell morphology and NBT reduction test, it is concluded that low-dose DNR could have inhibitory and cytotoxic effects on the cells, and its inducing differentiation was not important.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1991年第4期346-348,共3页
Academic Journal of Second Military Medical University
关键词
柔红霉素
白血病
HL-60细胞
daunorubicin
low-dose chemotherapy
leukemia
HL-60 cells
proliferation
differentiation
