摘要
目的 比较跨膜型和分泌型TNF α对中性粒细胞生物学功能影响的异同 ,以探讨两型TNF在炎症中的作用。方法 通过吞噬、呼吸爆发、原位杂交等方法研究跨膜型和分泌型TNF α对中性粒细胞生物学功能的影响。结果 分泌型TNF α可促进中性粒细胞吞噬 (P <0 .0 0 1)、呼吸爆发功能 (P <0 .0 5 )及IL 1βmRNA水平的表达 ,对中性粒细胞产生NO无明显影响 ;跨膜型TNF α对中性粒细胞吞噬、呼吸爆发功能无明显影响 ,对IL 1βmRNA水平的表达仅有微弱的促进作用 ,但可明显促进中性粒细胞产生NO。结论 分泌型TNF α对中性粒细胞有明显的激活作用 ,而跨膜型TNF
Objective To compare the effect of transmembrane type tumor necrosis factor α (TM TNF α) and secreting type TNF α (S TNF α) on the biological functions of neutrophils so as to explore the role of the both type of TNF α in inflammation. Methods Effect of the two type of TNF α on the functions of neutrophils was examined by phagocytosis, respiratory burst and in situ hybridization assay. Results S TNF α was found to be able to promote neutrophil phagocytosis markedly ( P <0.001), induce a striking respiratory burst ( P <0.005) and enhance the expression of IL 1β mRNA in neutrophils, while TM TNF α showed little influence on them. TM TNF α caused a two fold increase in NO production by neutrophils ( P <0.001) while S TNF α in a wide dosage range(10 1?000U) failed to exert any effect on NO production. Conclusions These results suggested that the mechanisms of two type of TNF α may be explained as that S TNF α promotes the inflammation in early phase, while TM TNF α plays an important role in the regulation of inflammation.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2001年第4期453-455,共3页
Chinese Journal of Microbiology and Immunology
基金
:国家自然科学基金重点资助项目 (396 30 32 0 )
关键词
跨膜型
分泌型
肿瘤坏死因子
TNF-α
中性粒细胞
Transmembrane type tumor necrosis factor α
Secreting type tumor necrosis factor α
Neutrophils
Respiratory burst
NO
