摘要
利用丙酸杆菌和内毒素引起的肝坏死模型进行了鼠肿瘤坏死因子－α（ＴＮＦ－α）单克隆抗体阻断肝坏死发生的研究。其结果：（１）鼠ＴＮＦ－α单克隆抗体有效地中和或清除了小白鼠血中的ＴＮＦ－α；（２）投与鼠ＴＮＦ－α单克隆抗体后，２４小时死亡率为１７％，较对照组（８３％）明显降低；（３）血清转氨酶明显降低，被抑制在丙酸杆菌单独投与组小白鼠血清转氨酶的水平；（４）肝组织学未发现大片肝坏死。上述结果说明ＴＮＦ－α单克隆抗体可以阻断实验性肝坏死的发生，进一步证明了ＴＮＦ－α是肝坏死发生的重要因子。
We couducted an investigation on the protective effects of anti-mouse TNF
monoclonalantibody m-TNF MAB)in liver cell necrosis of mice induced by injection of Pro-
pionibacterium acnes and a small dose of lipopolysaccharide-endotoxin(LPS).The
resultswere:1.Serum TNF was totally neutralized by the TNF MAB;2.When m-TNF MAB
wasadministered 2 hrs before LPS injection,the mortality 24 hrs after the injection of LPS
was17% which was significantly lower than that of control mice(83%);3.Levels of serum
transaminase activity in mice treated with TNF MAB were significantaly lower than those
ofcontrol mice; 4.No massive hepatic cell necrosis was found.Based on these finding,we
mayconclude that anti-TNF MAB can provide some protection against massive hepatic
cellnecrosis and TNF is one of the major mediators of liver injury.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1994年第3期150-152,共3页
Chinese Journal of Digestion
基金
国家自然科学基金
关键词
肝坏死
肿瘤坏死因子
单克隆抗体
Liver necrosis
tumor necrosis factor
mouse Anti-TNF monoclonal.
