摘要
为了解阿奇霉素在体外杀灭弓形虫速殖子的效果及其机制 ,故将阿奇霉素 (0 .5～ 1.5mg/mL)加入小鼠腹水中 (含虫 1× 10 5个 /mL) ,用光镜和透射电镜观察 .并将药物作用 4h的速殖子接种于小鼠以观察虫体是否复苏 .结果发现经 1.5mg/mL阿奇霉素作用 30min ,即可见速殖子的棒状体后段中央部分被溶解 ,速殖子细胞膜相结构和子虫细胞膜断裂 .经 1.5mg/mL阿奇霉素作用 4h的虫体在小鼠体内未能复苏 .可见阿奇霉素在体外对弓形虫速殖子具有杀灭作用 ,可以抑制速殖子的侵袭力和繁殖 .该药的杀虫力与药物的浓度和作用时间呈正相关趋势 .
Aim:To study the anti-Toxoplasma gondii tachyzoite effect of Azithromycin in vitro and its mechanism.Method:After Azithromycin (final concentration 0.5～1.5*#mg/mL) was added into the ascites of experimentally infected mice for 30～240*#min,the effect was observed under light microscope and transmission electron microscope.In addition,mice were inoculated with the drug-treated tachyzoites for 4h.Results:Under the action of 1.5*#mg/mL,Azithromycin for 30*#min,the rhoptries lysed and the daughter cell membrance and membranate structures of tachyzoites appeared broken.The tachyzoites treated by 1.5*#mg/mL Azithromycin for 4h could not be recovered in the inoculated mice.Conclusion:Azithromycin could kill Toxoplasma gondii tachyzoites in vitro.The penetration and multiplication of the tachyzoites could be inhibited by Azithromycin.The toxoplasmacidal activity of the drug has positive correlation with the concentration and time of action of Azithromycin.
出处
《南京师大学报（自然科学版）》
CAS
CSCD
2001年第2期95-98,共4页
Journal of Nanjing Normal University(Natural Science Edition)
基金
江苏省科技厅社会发展项目<弓形虫病治疗方法的研究>课题的一部分(编号为苏BS96 0 42 )
江苏省农林厅部分经费资助
关键词
阿奇霉素
弓形虫
速殖子
超微结构
体外实验
azithromycin
toxoplasma gondii
tachyzoite
in vitro
ultrastructure
