摘要
目的 研究弓形虫感染对雄性小鼠睾丸组织细胞周期的影响。方法 应用流式细胞术检测弓形虫感染10 3/ ml、10 4 / ml、10 6 / ml3个剂量组小鼠睾丸组织细胞周期,并设立生理盐水和环磷酰胺对照组。结果 不同剂量的弓形虫感染均可使小鼠睾丸组织细胞各时相的细胞百分数发生变化,并随着剂量的增加,G0 / G1 、S时相的细胞百分数明显减少,与阴性对照组比较差异有显著性(P<0 .0 5 )。G2 / M时相的细胞百分数逐渐增多,各剂量组与阴性对照组比较差异有显著性(P<0 .0 5 )。结论 弓形虫感染可能抑制小鼠睾丸细胞的DNA合成,可引起G2 期细胞阻滞,从而使细胞的有丝分裂延伸。
Objective To study the effects of Toxoplasma gondii infection on the testis cel l cycle of male mice. Methods The testis cell c ycle of male mice infected with Toxoplasma gondii was measured by the flow cytometry, and the physiological saline group and th e cyclophusphamide group were set as the control. Results The different doses of Toxoplasma gondii changed the testicle cell c ycle. The cells in G 0/G 1, S phase decreased with the increased dose of Toxoplasma gondii. There were significant differ ence s for the 103/ml, 104/ml, 106 /ml of Toxoplasma gondii groups compared with negative control group (P < 0.05)。The accounts of cells in G 2/M increased and there were significant dif ferences between the experimental groups and negative control( P<0.05)。 Conclusion Toxoplasm a gondii infection may inhibit DNA synthesis of testicle cells, cause the G 2 block and delay the mitosis of testicle cells.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2005年第2期130-132,共3页
Chinese Journal of Schistosomiasis Control
基金
江苏省计划生育委员会基金资助 (JS2 0 0 1-0 4)
关键词
弓形虫感染
雄性小鼠
细胞周期
睾丸
Toxoplasma gondii i nfection
Male mice
Cell cycle
Testicle
